Words similar to mini-gene
Example sentences for: mini-gene
How can you use “mini-gene” in a sentence? Here are some example sentences to help you improve your vocabulary:
To develop a transgenic model with high-level expression of hCAR on murine T cells, a transgene was constructed based on a human CD2 mini-gene described previously [ 16 ] (Fig.
We describe here three new transgenic (Tg) mouse lines in which expression of human CAR with a truncated cytoplasmic domain (hCARΔcyt) is limited to thymocytes and lymphocytes under direction of a human CD2 mini-gene [ 16 ] . The three lines are similar in having transgene expression restricted to the lymphoid lineage, but differ in the pattern of hCARΔcyt expression profiles within lymphoid populations.
This mini-gene includes the 5' promoter region of the hCD2 gene as well as the hCD2 LCR, which is reported to direct transgene expression to T cells in a position-independent, copy number-dependent manner [ 17 18 ] . Since the function of CAR is undefined, and deletion of the cytoplasmic tail of hCAR does not adversely affect adenoviral transduction [ 13 19 ] , a cDNA encoding a cytoplasmic tail-truncated variant of hCAR (hCARΔcyt) was generated and inserted into the CD2 mini-gene to eliminate any potential signaling capacity of hCAR.
In agreement with previous studies using human CD2 mini-gene expression cassette [ 16 17 18 ] , the truncated hCAR transgene was expressed in all cells of the T cell lineage, and except for transgenic line 6 (hCARΔcyt.CD2.
The resulting fragment (hCARΔcyt) was restriction endonuclease digested and subcloned into the EcoRI/SmaI site of the VA human CD2 mini-gene containing the human CD2 promoter and 3' LCR [ 16 ] . Automated sequencing (Applied Biosystems, Foster City, CA; University of Alabama at Birmingham Department of Microbiology sequencing facility) of the entire construct confirmed its accuracy.
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