Example sentences for: mini-gels

How can you use “mini-gels” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • A Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), which transfers in a horizontal apparatus with a platinum-coated titanium plate as anode and a stainless steel plate as cathode, was used to transfer the protein from the mini-gels to nitrocellulose membranes sandwiched between Extra Thick Blot Absorbent Filter Paper (Bio-Rad) in about 15 to 30 min.


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