Words similar to midasin

Example sentences for: midasin

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In both midasin and dynein, the evolutionary fusion of the six AAA protomers into a single polypeptide has permitted the individual protomers in the hexameric assembly to acquire substantial structural and functional specialization.
The sequence of the human midasin gene has been determined from PCR-amplified fragments of cDNA from human testis (Clontech).
The 3'-end of the coding region of the midasin gene was fused to a tag encoding 3× hemagglutamin (HA) by using the PCR-mediated cassette procedure of Longtine and colleagues [ 60 ] . The PCR primers used to generate the cassette were 5'-ACTGATTTTG CGTCAATACT TTACAGACCT GGCATCCAGC CGGATCCCCG GGTTAATTAA-3' and 5'-TCGTGTAGTA AACCTCCTCT TCTTGGTTTT CACGATATAC GAATTCGAGC TCGTTTAAAC-3'.
Differential centrifugation of the homogenate showed that the midasin remained in the supernatant fraction upon centrifugation at 2,000 × g for 2 min, but mostly passed into the pellet fraction upon centrifugation at 18,000 × g for 20 min.
The midasin signal covered a slightly larger area than the DAPI signal, and sometimes appeared granular or punctate.