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Example sentences for: midasin

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In Saccharomyces, midasin (Mdn1p) is encoded as a predicted polypeptide of 4910 amino acids (accession S64942; MM 560 kDa).
The M-domain of midasin is a highly conserved domain of ~280 residues (30 kDa) located at the COOH-terminus of the protein.
The N-terminal domain is the least conserved region of the midasin molecule (Table 1).
In order to confirm the yeast database entry indicating that the MDN1 (YLR106C) gene is essential for viability in yeast, we performed tetrad analysis of the heterozygous midasin knock-out strain CMEY072(HE) (Mat a/α ura3-52/ura3-52 his3D1/his3D1 leu2-3_112/leu2-3_112 trp1-289/trp1-289; MDN1(4,14727)::kanMX4/MDN1) obtained from EuroScarf.
The early evolutionary divergence of the different major branches of the AAA family makes it difficult to evaluate the phylogenetic relationships among them [ 45 ] . However, by restricting the analysis to residues in the more highly conserved regions of the AAA structure that interact directly with ATP, we have been able to improve the signal to noise ratio sufficiently to probe the phylogenetic relationship of midasin and dynein to representative members in other branches of the AAA family.