Words similar to midasin

Example sentences for: midasin

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The 3'-end of the coding region of the midasin gene was fused to a tag encoding 3× hemagglutamin (HA) by using the PCR-mediated cassette procedure of Longtine and colleagues [ 60 ] . The PCR primers used to generate the cassette were 5'-ACTGATTTTG CGTCAATACT TTACAGACCT GGCATCCAGC CGGATCCCCG GGTTAATTAA-3' and 5'-TCGTGTAGTA AACCTCCTCT TCTTGGTTTT CACGATATAC GAATTCGAGC TCGTTTAAAC-3'.
It is notable that the functionally important Asn in sensor 1 and Arg in sensor 2 [ 15 ] are present and invariant among organisms in all protomers of midasin, except AAA1 and AAA6.
Subsequent sporulation and tetrad dissection yielded 4 viable spores from most tetrads, indicating that the HA-tagged midasin is able to function and maintain viability in a haploid strain.
Tagging of the midasin gene in yeast
The early evolutionary divergence of the different major branches of the AAA family makes it difficult to evaluate the phylogenetic relationships among them [ 45 ] . However, by restricting the analysis to residues in the more highly conserved regions of the AAA structure that interact directly with ATP, we have been able to improve the signal to noise ratio sufficiently to probe the phylogenetic relationship of midasin and dynein to representative members in other branches of the AAA family.