Example sentences for: midas-containing

How can you use “midas-containing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This basic domain organization shows a striking parallel to that of magnesium chelatase, a heterotrimeric enzyme containing BchD, BchI and BchH subunits, that performs ATP-dependent insertion of Mg 2+into the protoporphyrin IX ring in the course of chlorophyll biosynthesis [ 49 ] . In particular, the BchD subunit resembles midasin in possessing a single AAA protomer close to its NH 2 -terminus, together with a short aspartate-glutamate-rich region and a MIDAS-containing domain at its carboxy-terminus.

  • Stripped to its fundamentals, the structure of midasin consists of a pseudo-hexameric assembly of AAA protomers associated with a MIDAS-containing M-domain.

  • The ability of a MIDAS protein to bind its protein ligand is tightly linked with the presence of intact MIDAS motifs: mutations to the metal-coordinating residues in these motifs, weaken or eliminate ligand binding [ 35 36 ] . An additional set of MIDAS-like proteins, that includes the integrin β-chain [ 37 38 ] and the von Willebrand Factor A domain [ 27 ] , lacks one of the three motifs and appears to bind ligands in a somewhat different manner [ 27 39 40 ] . In midasin, the presence of all three MIDAS motifs in the M-domain, with the putative metal ion-binding residues in the motifs invariant in the available organisms, indicates that midasin belongs to the family of MIDAS-containing proteins.

  • The best-studied examples of MIDAS-containing domains in other proteins are the I-domains of integrin α-chains-α1, α2, α10, and α11, and the A-domains of von Willebrand factor [ 9 25 26 27 ] . In proteins whose structure has been determined to atomic resolution, the MIDAS-containing domain has a classic Rossman-fold, with a central hydrophobic β sheet flanked on both sides by amphipathic helices.

  • The overall alignment of the midasin M-domain from humans, yeast and Giardia with other MIDAS-containing proteins (three Mg-chelatases and the I-domains of three integrins) was created by first aligning the midasins, Mg-chelatases and integrins separately with the program T-Coffee [ 58 ] . The resultant three partial alignments were then combined by the profile alignment procedure of ClustalX, as above.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast