Words similar to midas-containing
Example sentences for: midas-containing
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The overall alignment of the midasin M-domain from humans, yeast and Giardia with other MIDAS-containing proteins (three Mg-chelatases and the I-domains of three integrins) was created by first aligning the midasins, Mg-chelatases and integrins separately with the program T-Coffee [ 58 ] . The resultant three partial alignments were then combined by the profile alignment procedure of ClustalX, as above.
The best-studied examples of MIDAS-containing domains in other proteins are the I-domains of integrin α-chains-α1, α2, α10, and α11, and the A-domains of von Willebrand factor [ 9 25 26 27 ] . In proteins whose structure has been determined to atomic resolution, the MIDAS-containing domain has a classic Rossman-fold, with a central hydrophobic β sheet flanked on both sides by amphipathic helices.
Stripped to its fundamentals, the structure of midasin consists of a pseudo-hexameric assembly of AAA protomers associated with a MIDAS-containing M-domain.
The ability of a MIDAS protein to bind its protein ligand is tightly linked with the presence of intact MIDAS motifs: mutations to the metal-coordinating residues in these motifs, weaken or eliminate ligand binding [ 35 36 ] . An additional set of MIDAS-like proteins, that includes the integrin β-chain [ 37 38 ] and the von Willebrand Factor A domain [ 27 ] , lacks one of the three motifs and appears to bind ligands in a somewhat different manner [ 27 39 40 ] . In midasin, the presence of all three MIDAS motifs in the M-domain, with the putative metal ion-binding residues in the motifs invariant in the available organisms, indicates that midasin belongs to the family of MIDAS-containing proteins.
This basic domain organization shows a striking parallel to that of magnesium chelatase, a heterotrimeric enzyme containing BchD, BchI and BchH subunits, that performs ATP-dependent insertion of Mg 2+into the protoporphyrin IX ring in the course of chlorophyll biosynthesis [ 49 ] . In particular, the BchD subunit resembles midasin in possessing a single AAA protomer close to its NH 2 -terminus, together with a short aspartate-glutamate-rich region and a MIDAS-containing domain at its carboxy-terminus.
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