Words similar to microsatellites
Example sentences for: microsatellites
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By optimizing the hybridization and washing steps, most of the AFLP fragments were found to harbor microsatellites after cloning and sequencing.
Since our algorithms are based on searching for clusters of common sites, microsatellites can score high, but tend not to be functional (for an exception see [ 9 ] ). We used the Tandem Repeat Remover [ 17 ] to mask microsatellites, with scoring parameters (2 5 5 75 20 20 500) (respectively; match, mismatch, indel scores; percentage priors for mismatch and indels; minimum score, and maximum length) which are as promiscuous as possible yet did not detect appreciable microsatellites in random sequences.
While screening the SSR-enriched, small-insert libraries can significantly reduce time and cost [ 11 ] . Enrichment can be realized by hybridizing SSR-containing fragments with biotin-labeled probes that are either captured by magnetic beads coated with streptavidin or fixed on a nitrate filter [ 12 ] . The eluted portion after removing non-hybridized DNA was highly enriched for microsatellites, with enrichment efficiency between 50-90% [ 13 ] .
This estimate assumes that only a single RI animal is sampled per line; a strategy that is appropriate for mapping SNPs, microsatellites and other Mendelian loci.
It seems likely that, as in the HLA region [ 32], there will be a limited number of ancestral haplotypes in the 1q41 region, and that these ancestral haplotypes will be identifiable by typing a dense map of microsatellites.