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Example sentences for: microsatellites
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While screening the SSR-enriched, small-insert libraries can significantly reduce time and cost [ 11 ] . Enrichment can be realized by hybridizing SSR-containing fragments with biotin-labeled probes that are either captured by magnetic beads coated with streptavidin or fixed on a nitrate filter [ 12 ] . The eluted portion after removing non-hybridized DNA was highly enriched for microsatellites, with enrichment efficiency between 50-90% [ 13 ] .
on peanut microsatellites [ 21 ] , in which five polymorphic markers from 26 primer pairs (19%) were identified using library screening method, our enrichment procedure was more efficient in identifying SSR markers, where 19 of 56 primer pairs (34%) showed a polymorphism.
Since our algorithms are based on searching for clusters of common sites, microsatellites can score high, but tend not to be functional (for an exception see [ 9 ] ). We used the Tandem Repeat Remover [ 17 ] to mask microsatellites, with scoring parameters (2 5 5 75 20 20 500) (respectively; match, mismatch, indel scores; percentage priors for mismatch and indels; minimum score, and maximum length) which are as promiscuous as possible yet did not detect appreciable microsatellites in random sequences.
It seems likely that, as in the HLA region [ 32], there will be a limited number of ancestral haplotypes in the 1q41 region, and that these ancestral haplotypes will be identifiable by typing a dense map of microsatellites.
The genotype data from the 11 microsatellites used in the study were analyzed using PEDCHECK and RELATIVE to identify errors in Mendelian inheritance and to confirm familial relationships [ S2, S3].
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