Words similar to microextensions
Example sentences for: microextensions
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Quick extraction with 0.2% Triton X-100 in MTSB (30 seconds, 2 times), followed by fixation with 1% glutaraldehyde also preserved microextensions well indicating that fragmentation or removal of microextensions by extraction with 0.2% Triton X-100 does not occur within 1 min.
Our experiments were focused on the analysis of microextensions and their cytoskeletal core by SEM and confocal laser scanning microscopy by searching for a new fixation protocol.
As expected, we show here that some of microextensions were fragmented by the standard Triton X-100 permeabilization method.
On the other hand, microextensions of cells prefixed with glutaraldehyde or DSP prior to extraction were very well preserved indeed (Figure 3Eand 3I).
Acetone also provided good preservation of microextensions even after preparing the cells for immunolabeling, whereas some of the microextensions were lost, when cells were fixed with other coagulant fixatives (Table 2).