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Example sentences for: microextensions

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Preservation and exposure of cytoskeleton in microextensions and ultramicroextensions
For example, when cells were fixed with 3.7% FA for 20 min and extracted with 0.2% TX100 for 5 min (Table 1), stress fibers were stained well with phalloidin, but microextensions were lost (Figure 3Aand 3B).
Acetone also provided good preservation of microextensions even after preparing the cells for immunolabeling, whereas some of the microextensions were lost, when cells were fixed with other coagulant fixatives (Table 2).
Among the coagulant fixatives, acetone produced the best results with respect to preservation of microextensions, staining of actin with either phalloidin or with monoclonal β-actin antibodies, and staining of microtubules with a monoclonal anti-β-tubulin reagent.
The excellent preservation of the cytoskeleton in microextensions of cells fixed with PFA/DOTMAC could be due to stabilization of the cytoskeleton in general or of actin filaments selectively.