Example sentences for: methanol

How can you use “methanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • When the cells were treated with DOTMAC lysis buffer, they did not detach and, therefore, lipids could be extracted directly from the cells on glass culture dishes with 1 ml of chloroform/methanol (2:1, v/v) after treatment with the detergent extraction buffer.

  • Sections were fixed for 1 minute in ice cold methanol; blocked for 45 minutes in blocking buffer (15% horse serum, 0.025% Triton, 1x PBS); incubated overnight at 4°C with anti-sarcospan (1:750) and anti-spectrin (1:100) antibodies in block buffer; washed 3 × for 15 minutes in wash buffer (0.

  • For Arp3 immunofluorescence, cells were post-fixed in methanol (-20°C) for 2 minutes.

  • Gels were incubated for 20 min in an aqueous solution of 50% methanol/10% acetic acid, followed by 10% ethanol/10% acetic acid for 20 min, and, finally, Amplify (Amersham) for 20 min.

  • Proteins were transferred onto nitrocellulose membranes (Genie blotter, Idea Scientific Company, Minneapolis, MN, USA) for 1 hour, at 24 V and 4°C, using transfer buffer composed of 25 mM Tris-HCl, 192 mM glycine and 10% methanol.


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