Example sentences for: methanol

How can you use “methanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cell monolayers were rinsed 3 times with PBS, fixed for 10 minutes in -20 oC methanol, and then air dried for 1 hr.

  • Fixation was performed by incubating samples for 10 min with cooled methanol at -20°C and washed with PBS three times.

  • Since the background fluorescence in live, cycling cells was too high to derive conclusions about Rad26-GFP localization (data not shown), we prepared cells using a technique previously described in a study of fission yeast chromatin binding proteins [ 42 ] . An asynchronous population of cycling cells was first permeabilized using Zymolyase (Seikagaku Corp., Tokyo), then extracted with 1% Triton X-100 and finally fixed with methanol.

  • At the end of the differentiation protocol (day 14), additional plates were stained to detect foci formation as follows: cells were washed with 1 × PBS, fixed in methanol for 1 minute, stained with bromophenol blue for 1 minute, then washed again with 1 × PBS.

  • Cells grown on coverslips were washed briefly in a phosphate-buffered saline (PBS) solution (pH 7.4, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM KH 2 PO 4 ) and then either fixed in 100% methanol at -20°C for 1 minute or at room temperature in a 4% formaldehyde (PFA) solution freshly made from paraformaldehyde (Electron Microscopy Sciences, Ft.


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