Example sentences for: metaphases

How can you use “metaphases” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The SCE analysis of this cell line was complicated by difficulty in finding chromosome spreads and by the small number of metaphases present in the cultures.

  • Fixed preparations were dropped onto clean glass slides and stained with 0.1 mg/ml acridine orange for 5 minutes, followed by a wash in running water for 2 minutes and a 1 minute incubation in Sorensen's buffer (67 mM KH 2 PO 4 + 67 mM Na 2 HPO 4 ). The wet slides were covered with glass coverslips and metaphases photographed immediately with a Zeiss Axioskop fluorescence microscope using the FITC filter and a 100× objective.

  • 3 was hybridized to patient's metaphases and showed signal on both ends of the abnormal X chromosome (as well as the normal X chromosome) confirming the G-band impression that both ends of this chromosome was comprised of Xp.

  • Fluorescence in situ hybridization (FISH) was performed on metaphases from cultured lymphocytes of two patients with abnormal G-band findings.

  • The SCE assay is therefore biased towards resolved or more corrected metaphases and it is likely that the induced ΔH1 SCE frequency is actually higher than reported here.


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