Example sentences for: luciferin

How can you use “luciferin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The luc reporter was driven by a portion of the period (per) gene (its 5'-flanking sequences and those encoding the N-terminal 2/3 of the protein) in some cases or the 5'-flanking sequences of the timeless (tim) gene in the others; these molecular constructs were introduced into the D. melanogaster genome by germline transformation [ 32 18 33 ] . Reporter activity was sampled from an enzymatic reaction (luciferase oxidizing luciferin, present in the medium surrounding the antennae); the reaction produces a bioluminescent signal that is measured in counts per second once each hour [ 13 ] . One genetically based comparison made in this study involved the effects of the (normal) cry +allele compared with that of cry b, tested in LD 12:12 (12 hours of light followed by 12 hours of darkness, over the course of about 5-6 cycles per specimen).

  • Linear as well as nonlinear trends in the data come into play in experiments using luciferase reporter technology [ 19 20 ] . Such artifacts were shown (in one substrate replenishment experiment) to be the result of inevitable depletion of luciferin from the medium surrounding the tissues over time [ 19 ] . Removal of these trends quantitatively clarifies the record of bioluminescent activity with regard to any rhythmicity that might be present [ 27 ] . The normalization procedure we use (see Figure 1and [ 27 ] for more detail) exposes the rhythm (if one is present) in a manner that is quantifiable without reference to the type of measurement that has been employed (for example, counts-per-second metric on the ordinate of the relevant plots becomes dimensionless).

  • This both corrects for the damping evident in 5c (a result in this case of luciferin depletion in the medium) and reveals that the rhythm is actually just as robust later in the experiment, even though it appears to be damping prior to normalization.


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