Words similar to luciferase
Example sentences for: luciferase
How can you use “luciferase” in a sentence? Here are some example sentences to help you improve your vocabulary:
In all experiments, luciferase activities were normalized with the renilla luciferase activity expressed from the pCMV-RL vector.
Such concerns motivated us to develop a set of tools for the analysis of circadian rhythms [ 27 ] . Here we apply two key features of this analytic package that were unavailable for our earlier report on the effects of cry b in the periphery [ 23 ] . First is a method to eliminate nonlinear trends in the data, a potential source of artifact that could affect whether luciferase activity is scored as rhythmic or arrhythmic.
Luciferase reporter plasmids containing specific responsive elements for AR, GR, and PR (GRE-TATA-luciferase) and ER (ERE-TATA-luciferase) have been described elsewhere [ 29 30 ] ; another androgen-responsive reporter contained the fragment -286/+28 of the rat Probasin (PB) promoter, and was obtained from Dr. R. Matusik, Vanderbilt University, TN [ 31 ] . An expression plasmid for wild-type hAR was kindly provided by Dr. C. Chang, University of Rochester, NY.
Such nonlinear trends are common in luciferase assays and are likely to be caused by the depletion of substrate from the medium over time [ 18 10 ] . We use digital filters to remove non-linear trends from signals and also to smooth them when they contain high frequency noise (see below).
When A549 cells were treated with ISIS 15534 and then dexamethasone, the effect was additive, with maximal dexamethasone induced luciferase activity ~ 10 times greater than the maximal dexamethasone-induced response attainable in the presence of PP5 [ 25].
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