Words similar to locus
Example sentences for: locus
How can you use “locus” in a sentence? Here are some example sentences to help you improve your vocabulary:
In Riley et al . [ 17 ] , the authors theorize that the duplication of the CHRNA7 locus is a human-specific event because of the high sequence homology of the duplicated segment to the donor segment in 15q13.
Using restriction-length-fragment polymorphism (RFLP) and RFLP-derived cleaved amplified polymorphic sequence (CAPS) markers (Figure 1) based on previously published tomato genetic and RFLP maps [ 8], initial studies mapped the dgt locus to a region of around 0.8 cM near the bottom of the long arm of tomato chromosome 1. Of 1,308 backcross (BC 1 ) individuals screened, 10 plants were determined to be recombinant between markers TG269 and CT190, whereas no plants were found to be recombinant between TG389 and dgt (Figure 1).
The ndr1-1 deletion was detected by Southern blotting done essentially as previously described [ 6 ] . To make a radiolabeled probe, part of the NDR1 locus was amplified using ndr1-1 genomic DNA as template.
A full-length L1 insertion in the genome is approximately 6,000 nucleotides long and consists of a 5' untranslated region (UTR), two open reading frames (ORFs), and a 3' UTR terminating in a poly(A) tail [ 11 ] . The second ORF of L1 encodes three domains critical for L1 propagation: endonuclease (EN) [ 12 ] , reverse transcriptase (RT) [ 13 14 ] , and a 3' terminal zinc-finger-like domain [ 15 ] . The EN and RT nick a target site in DNA and reverse transcribe L1 RNA, respectively, to integrate into a new genomic locus [ 12 16 17 18 ] ; this process is known as target-site-primed reverse transcription (TPRT).
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
Loading...