Example sentences for: lm-pcr

How can you use “lm-pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • LM-PCR directed at MAC telomeres

  • The first protocol uses a ligation-mediated PCR strategy (LM-PCR) in which ChIP DNA is ligated to short DNA linkers, and then PCR amplified using the linker sequence for priming [ 4 ] . The second uses a random amplification strategy (R-PCR) in which template DNA is first copied using a specialized primer with degenerate 3' sequence and known 5' sequence, and then PCR amplified using the known 5' sequence for priming [ 5 8 9 ] . In both cases, the PCR steps yield quantities of nucleic acid sufficient for microarray analysis.

  • adapted LM-PCR to this application, and generated the data.

  • LM-PCR has been used in several studies, including a systematic genome-wide location analysis of yeast transcriptional activators [ 10 ] , and an unbiased search for E2F binding sites in a subset of human promoters [ 11 ] . Studies employing the R-PCR approach include genome-wide analyses of silencing enzymes [ 12 ] and histone modifications [ 6 7 ] in yeast, and a mapping of GATA-1 binding sites within the human β-globin locus [ 13 ] .

  • LM-PCR, ligation-mediated polymerase chain reaction; MAC, macronucleus; MIC, micronucleus; TASs, telomere addition sites; CR-R, CR-L, Common Region-Right, -Left.


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