Example sentences for: llwo
How can you use “llwo” in a sentence? Here are some example sentences to help you improve your vocabulary:
Uninduced and induced LLWO2F cells (10 × 100 mm dishes) were washed 3-times with ice-cold serum-free DMEM containing 10 ug/ml cycloheximide.
Lysates from induced LLWO2F cells were combined with GST-alone- or GST-PNUTS-loaded glutathione Sepharose beads.
As shown in Figure 3, there is a predominant nuclear localization of the hemagglutinin epitope (panel A) compared to the low-level cytoplasmic and nuclear background reactivity seen in the uninduced LLWO2F control cells (panel B).
Induction of GST-PNUTS fusion protein synthesis and isolation by affinity chromatography using glutathione-Sepharose beads (Pharmacia) was performed as described previously [ 32 ] . Induced LLWO2F cell extracts were then incubated for 1 hour at 4°C with recombinant GST or GST-PNUTS fusion protein bound to glutathione-Sepharose.
Negative control LLWO2F cells were not induced with doxycycline.
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