Example sentences for: llwo
How can you use “llwo” in a sentence? Here are some example sentences to help you improve your vocabulary:
A time course of induction by doxycycline was then carried out in stable clone LLWO2F.
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
As shown in Figure 3, there is a predominant nuclear localization of the hemagglutinin epitope (panel A) compared to the low-level cytoplasmic and nuclear background reactivity seen in the uninduced LLWO2F control cells (panel B).
To address this, we tested the phosphatase activity of LLWO2F whole-cell lysate using phosphorylase a as the substrate.
LLWO2F cells were induced with doxycycline (2 ug/ml) for 24 hr.
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