Example sentences for: llwo
How can you use “llwo” in a sentence? Here are some example sentences to help you improve your vocabulary:
Uninduced and induced LLWO2F cells (10 × 100 mm dishes) were washed 3-times with ice-cold serum-free DMEM containing 10 ug/ml cycloheximide.
LLWO2F cells were induced with doxycycline (2 ug/ml) for 24 hr.
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
To address this, we tested the phosphatase activity of LLWO2F whole-cell lysate using phosphorylase a as the substrate.
Induction of GST-PNUTS fusion protein synthesis and isolation by affinity chromatography using glutathione-Sepharose beads (Pharmacia) was performed as described previously [ 32 ] . Induced LLWO2F cell extracts were then incubated for 1 hour at 4°C with recombinant GST or GST-PNUTS fusion protein bound to glutathione-Sepharose.
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