Example sentences for: llwo
How can you use “llwo” in a sentence? Here are some example sentences to help you improve your vocabulary:
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
First, immunocomplexed 6His-HA-PP1α was tested for its ability to dephosphorylate phosphorylase a . Phosphorylated on a single serine residue, this substrate is often used to monitor the biochemical activity of PP1 in vitro [ 28 ] . As shown in Figure 5, greater phosphatase activity is detected in immunoprecipitates from induced LLWO2F cell lysates compared to uninduced.
Uninduced and induced LLWO2F cells (10 × 100 mm dishes) were washed 3-times with ice-cold serum-free DMEM containing 10 ug/ml cycloheximide.
LLWO2F cell extracts (50 ug) were immunoprecipitated with approximately 1 ug of isoform-specific rabbit antiserum to PP1α as previously described [ 38 ] . Immunoprecipitation with 1 ug of normal rabbit serum served as a negative control.
Lysates from induced LLWO2F cells were combined with GST-alone- or GST-PNUTS-loaded glutathione Sepharose beads.
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