Example sentences for: llwo
How can you use “llwo” in a sentence? Here are some example sentences to help you improve your vocabulary:
To address this, we tested the phosphatase activity of LLWO2F whole-cell lysate using phosphorylase a as the substrate.
Lysates from induced LLWO2F cells were combined with GST-alone- or GST-PNUTS-loaded glutathione Sepharose beads.
Uninduced and induced LLWO2F cells (10 × 100 mm dishes) were washed 3-times with ice-cold serum-free DMEM containing 10 ug/ml cycloheximide.
First, immunocomplexed 6His-HA-PP1α was tested for its ability to dephosphorylate phosphorylase a . Phosphorylated on a single serine residue, this substrate is often used to monitor the biochemical activity of PP1 in vitro [ 28 ] . As shown in Figure 5, greater phosphatase activity is detected in immunoprecipitates from induced LLWO2F cell lysates compared to uninduced.
Induction of GST-PNUTS fusion protein synthesis and isolation by affinity chromatography using glutathione-Sepharose beads (Pharmacia) was performed as described previously [ 32 ] . Induced LLWO2F cell extracts were then incubated for 1 hour at 4°C with recombinant GST or GST-PNUTS fusion protein bound to glutathione-Sepharose.