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Example sentences for: lhcps
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Molecular modeling suggested that this 'retention' motif - ExxHxR in the first and ExxNxR in the third membrane-spanning region - within all LHCPs and related proteins provides two ligands for Chl, an ion-pair between Glu (E) and Arg (R) and the sidechain of either His (H) or Asn (N).
Experiments with the model alga Chlamydomonas reinhardtii [ 11 ] showed that LHCPs were not detectably imported into the chloroplast in the absence of Chl synthesis and instead accumulated outside of the chloroplast in the cytosol and in vacuoles [ 12 ] . High concentrations of chloramphenicol caused strong suppression of total Chl synthesis when dark-grown algal cells were exposed to light, possibly by inhibition of Mg-chelatase [ 13 ] in addition to chloroplast protein synthesis.
2b, immunoelectron microscopy detected LHCPs along the chloroplast envelope.
The most electronegative ligand in LHCPs is the sidechain of His.
An important role of Chl b was further revealed by experiments in which newly synthesized LHCPs were detected by pulse-labeling in Chl b -less mutant plants but the proteins were not recovered in chloroplasts isolated from these plants [ 4 ] . These Chl b -less plants did not accumulate several of the LHCPs, in particular Lhcb1, Lhcb6 and Lhca4 [ 5 ] . Chl b was not detected in plants exposed to intermittent light (cycles of 2 min of light and 98 min of darkness), which accumulated only small amounts of Chl a and thylakoid membranes [ 6 7 ] . Wild-type plants treated in this way accumulated only one LHCP (Lhcb5), while Chl b -less mutants exposed to intermittent light lacked all LHCPs [ 8 ] . In complementary fashion, Chl b did not accumulate when synthesis of LHCPs was inhibited [ 9 ] . When bean plants exposed to intermittent light were treated with chloramphenicol to inhibit synthesis of proteins on chloroplast ribosomes, Chl
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