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Example sentences for: lhcps
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An important role of Chl b was further revealed by experiments in which newly synthesized LHCPs were detected by pulse-labeling in Chl b -less mutant plants but the proteins were not recovered in chloroplasts isolated from these plants [ 4 ] . These Chl b -less plants did not accumulate several of the LHCPs, in particular Lhcb1, Lhcb6 and Lhca4 [ 5 ] . Chl b was not detected in plants exposed to intermittent light (cycles of 2 min of light and 98 min of darkness), which accumulated only small amounts of Chl a and thylakoid membranes [ 6 7 ] . Wild-type plants treated in this way accumulated only one LHCP (Lhcb5), while Chl b -less mutants exposed to intermittent light lacked all LHCPs [ 8 ] . In complementary fashion, Chl b did not accumulate when synthesis of LHCPs was inhibited [ 9 ] . When bean plants exposed to intermittent light were treated with chloramphenicol to inhibit synthesis of proteins on chloroplast ribosomes, Chl
b and LHCPs accumulated in parallel with no increase in synthesis of total Chl [ 10 ] . These results indicate that photosystem I and II core complex proteins, which are synthesized in the chloroplast, compete effectively with LHCP for small amounts of Chl a made under these conditions, and that Chl b does not accumulate until sufficient Chl a is made to satisfy core complexes.
The availability of Chl b thus strongly regulates import of LHCPs as well as assembly and eventual accumulation of light-harvesting complexes.
2b, immunoelectron microscopy detected LHCPs along the chloroplast envelope.
The initial accumulation of LHCPs in the chloroplast envelope implies that Chl interacts with these proteins, likely by binding to the conserved motif in the first membrane-spanning region (helix-1) [ 25 26 ] when transit through the envelope is initiated.