Words similar to lexa
Example sentences for: lexa
How can you use “lexa” in a sentence? Here are some example sentences to help you improve your vocabulary:
A deliberate search for activator peptides revealed that, although C-terminal peptide fusions to the lexA DNA binding domain had a higher probability of activating transcription, sequences inserted within the structure of a green fluorescent protein (GFP) scaffold also functioned as activators.
However, a peptide selected in the context of the amino terminal portion of GFP functioned effectively when this GFP segment was removed and the peptide was grafted directly onto lexA.
For the yeast two-hybrid screen, full-length Smad1 was subcloned into BamH1/Not1 sites of PEG202 by a PCR method, resulting in the inframe fusion between the LexA DNA binding domain (1-202) and Smad1.
One of the peptide activators from the lexA-GFP/peptide library, derived from a frameshift in the insert, was tested for its ability to activate transcription when separated from the GFP structure and directly fused to the lexA binding domain.
For example, this has been carried out with MAP kinase deletion mutants after pheromone treatment [ 10], deletions of effectors of the Tor proteins after rapamycin treatment [ 11], and lexA deletions after UV irradiation [ 24].