Words similar to lexa
Example sentences for: lexa
How can you use “lexa” in a sentence? Here are some example sentences to help you improve your vocabulary:
Yeast strain yVT87 (EGY48 (Clontech) MATa, ura3, his3, trp1, leu2::lexA6op- LEU2 ) was transformed by the method of Gietz and Schiestl (Agatep, R., R.D.
Because Gal4-GFP fusions are unstable, we were unable to analyze the GFP/peptide insertions identified in the lexA hybrid proteins in the context of the Gal4BD (not shown).
A deliberate search for activator peptides revealed that, although C-terminal peptide fusions to the lexA DNA binding domain had a higher probability of activating transcription, sequences inserted within the structure of a green fluorescent protein (GFP) scaffold also functioned as activators.
A second library in which 15 residue-long random peptides were fused to the C-terminus of the lexA binding domain was also constructed.
The recruitment model for transcriptional activation stipulates that eukaryotic transcription is driven mainly by recruitment of preformed RNA polymerase holoenzyme to specific sites on the DNA [ 3 ] . According to this model, peptides may bind one of numerous proteins that guide holoenzyme to the lexA binding site.
Loading...