Example sentences for: lexa

How can you use “lexa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A deliberate search for activator peptides revealed that, although C-terminal peptide fusions to the lexA DNA binding domain had a higher probability of activating transcription, sequences inserted within the structure of a green fluorescent protein (GFP) scaffold also functioned as activators.

  • However, a peptide selected in the context of the amino terminal portion of GFP functioned effectively when this GFP segment was removed and the peptide was grafted directly onto lexA.

  • For the yeast two-hybrid screen, full-length Smad1 was subcloned into BamH1/Not1 sites of PEG202 by a PCR method, resulting in the inframe fusion between the LexA DNA binding domain (1-202) and Smad1.

  • One of the peptide activators from the lexA-GFP/peptide library, derived from a frameshift in the insert, was tested for its ability to activate transcription when separated from the GFP structure and directly fused to the lexA binding domain.

  • For example, this has been carried out with MAP kinase deletion mutants after pheromone treatment [ 10], deletions of effectors of the Tor proteins after rapamycin treatment [ 11], and lexA deletions after UV irradiation [ 24].


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