Words similar to leupeptin
Example sentences for: leupeptin
How can you use “leupeptin” in a sentence? Here are some example sentences to help you improve your vocabulary:
They were then lysed in a protein kinase lysis buffer [50 mM HEPES, pH 7.5, 150 mM NaCI, 1.5 mM MgCI2, 1 mM EGTA, 1 mM sodium vanadate, 50 mM sodium fluoride, 20 mM beta-glycerophosphate, 10% (vol/vol) glycerol, 1% (vol/vol) Triton X-100, and protease inhibitors (1 mM PMSF, 1 ug/ml pepstatin A1, 1 ug/ml leupeptin, and 1 ug/ml aprotinin].
Cells were scraped into ice-cold lysis buffer: 50 mM HEPES, pH 7.4, 150 mM sodium chloride, 1.5 mM magnesium chloride, 10% glycerol, 1% Triton X-100, 1 mM EGTA, 200 μM sodium vanadate, 10 mM tetrasodium pyrophosphate, 100 mM sodium fluoride, 10 μg/ml leupeptin, and 10 μg/ml aprotinin.
Protein lysates from whole cells and tissues were prepared with a buffer, which contained 50 mM Tris, pH 7.5, 150 mM NaCl, 1% (v/v) Nonidet P40 (NP40), 2 mM 4-(2-aminoethyl)benzenesulphonyl fluoride, 1 μg/ml leupeptin, 2 μg/ml aprotinin and 2 μg/ml pepstatin.
The frozen cell pellets from 500 ml spinner flask culture were lysed by sonication in Buffer A (50 mM Tris-HCL pH 8, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 μg/ml leupeptin, pepstatin, and E64, and 1 mM PMSF) containing 450 mM NaCL and 1% NP40.
The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.
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