Words similar to leupeptin

Example sentences for: leupeptin

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P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
Yeast cytosol (from 541,000 × g spins) containing wild-type and mutant versions of Boi1-PH were prepared and stored as described for the proteolysis assay, except that the protease inhibitors leupeptin, benzamidine, and EGTA were included.
Cell were harvested by low-speed centrifugation and resuspended in lysis buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 0.5 M NaCl, 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT).
For immunoprecipitations, labeled cellular proteins were extracted with lysis buffer (1% of the indicated detergent, 20 mM Hepes, pH 7.4, 150 mM NaCl, 2 mM PMSF, 10 μg/ml leupeptin, 10 μg/ml aprotinin, 2 mM MgCl2, 0.5 mM CaCl2) for 60 minutes at 4°C.
Briefly, retinae were isolated with homogenization buffer (HB, 0.32 mM sucrose, 2 mM Hepes, pH 7.4, 1 mM EGTA, 2 μg/ml leupeptin and 2 μg/mg Aprotinin) and homogenized.