Words similar to lactacystin

Example sentences for: lactacystin

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Moreover, immunoblot analysis of the His 6 Rab24(D123I) isolated from cells treated with lactacystin (to block degradation of poly-ubiquitinated proteins by the 26S proteasome) showed no detectable ubiquitin associated with the protein, although the same antibody detected multiple ubiquitinated polypeptides in the whole-cell lysate (Fig.
The incomplete rescue of SNIP1 level by lactacystin suggests the involvement of additional mechanisms other than 26S proteasome in SNIP1 degradation.
The effect of lactacystin is specific, since the control reporter gene β-Gal was not inhibited by lactacystin (data not shown).
For example, the protein levels of Smad1, Smad4 and Az were all sensitive to lactacystin (Fig.
20 μM lactacystin (Sigma, St. Louis, MO) was added to the medium 18 h after transfection and cells were incubated with the proteasome inhibitor for 4 h prior to harvest.