Words similar to lacos
Example sentences for: lacos
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Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.
As the lac repressor variant that we used binds to the operator sites as dimers, we should be able to inhibit its binding to the LacOs of the tagged loci by adding IPTG [ 27].
If the binding is specific to the LacOs inserted in the genome, the observed fluorescence spots should be suppressed by the addition of IPTG.
If the system functions properly, we should be able to detect the tagged loci as bright spots above the GFP background signals in nuclei of the lines EL702C and E, and should not detect any spot in line EL700S because of the absence of LacOs . Bright spots were clearly observed above the diffused GFP fluorescence in nuclei observed 24 h after induction in lines EL702C and E, whereas significant fluorescence spots above the diffuse background signals were not observed in EL700S (Figure 4, rows B).
As Belmont and co-workers [ 14, 25] reported that the LacOs is unstable in a recombination-competent bacterial strain, we are concerned with the stability of LacOs in the Agrobacterium strain that is used to transform Arabidopsis . Thus, we examined the stability of LacOs in the Agrobacterium tumefaciens strain GV3101/MP90 by Southern blot analysis after transformation.
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