Words similar to lacos

Example sentences for: lacos

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This latter was transformed with pEL700, which is identical to pEL702 except for the absence of LacOs . As pEL700 does not contain the LacOs , the transgenic line EL700S can be used as a negative control for specific association of GFP-LacI/NLS with the LacOs in our experimental system.
Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.
If the binding is specific to the LacOs inserted in the genome, the observed fluorescence spots should be suppressed by the addition of IPTG.
After 48 hours, the detection levels did not change significantly and comparison between the three lines revealed that the Dex-inducible system in each line performed similarly with and without truncated LacOs , and minimal leakiness of the promoter was observed before induction.
Both western blot analysis and microscopy studies confirmed that the LacOs does not interfere with glucocorticoid-inducible expression.