Words similar to lacos
Example sentences for: lacos
How can you use “lacos” in a sentence? Here are some example sentences to help you improve your vocabulary:
After we obtained several independent lines that grew on selection plates containing hygromycin, we used Southern blot analysis to determine the size of the LacOs and the relative copy number of the inserted T-DNAs in the transformed lines.
These results indicated that the LacOs could be truncated before or during integration into the Arabidopsis genome, but the integrated genes were subsequently stable in the plant genome.
If the system functions properly, we should be able to detect the tagged loci as bright spots above the GFP background signals in nuclei of the lines EL702C and E, and should not detect any spot in line EL700S because of the absence of LacOs . Bright spots were clearly observed above the diffused GFP fluorescence in nuclei observed 24 h after induction in lines EL702C and E, whereas significant fluorescence spots above the diffuse background signals were not observed in EL700S (Figure 4, rows B).
The cultures in which full-length or near full-length LacOs was found to be maintained were then increased to 500 ml and incubated at 28°C for 12 h before use for Arabidopsis transformation.
Pst I digestion of genomic DNA from the transgenic lines, which should release the full-length LacOs (10.