Words similar to lacos

Example sentences for: lacos

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Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.
After 48 hours, the detection levels did not change significantly and comparison between the three lines revealed that the Dex-inducible system in each line performed similarly with and without truncated LacOs , and minimal leakiness of the promoter was observed before induction.
We speculate that a large T-DNA region (approximately 17 kb) and/or the presence of a large number of tandem repeats of LacOs in the T-DNA region may contribute to the low efficiency of gene transfer.
We then examined nucleus labeling by GFP after Dex treatment in homozygous transgenic plants from lines EL702C and E (with LacOs ), and line EL700S (without LacOs ) by fluorescence microscopy.
Pst I digestion of genomic DNA from the transgenic lines, which should release the full-length LacOs (10.