Words similar to laci
Example sentences for: laci
How can you use “laci” in a sentence? Here are some example sentences to help you improve your vocabulary:
Anti-GFP polyclonal antibodies (Clontech) were used at 1/5,000 dilution to detect mGFP5-LacI/NLS proteins.
The E. coli , strain TG1 (genotype = K12Δ (lac-pro), supE, thi, hsdΔ5/F' [traD36, proAB , lacI q, lacZΔM15 ]) was obtained from Pharmacia (Piscataway NJ).
The copy numbers of insertions in these lines were estimated with restriction digests of genomic DNA using Bgl II and Hind III in addition to Pst I, followed by Southern blot analysis using the LacI/NLS fragment as a probe (Figures 1, 2).
The second was the random primer-labeled LacI/NLS . Membranes were prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C in ExpressHyb hybridization solution and washed at 60°C in washing solution.
A gene encoding a plant-optimized GFP variant, mGFP5 [ 24], was placed in frame with a LacI -SV40 nuclear localization signal fusion ( GFP-LacI/NLS ) [ 25] and then subcloned into the vector pTA7002 under the control of the glucocorticoid receptor (GR)-responsive promoter near the left border.
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