Example sentences for: laci

How can you use “laci” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Anti-GFP polyclonal antibodies (Clontech) were used at 1/5,000 dilution to detect mGFP5-LacI/NLS proteins.

  • The E. coli , strain TG1 (genotype = K12Δ (lac-pro), supE, thi, hsdΔ5/F' [traD36, proAB , lacI q, lacZΔM15 ]) was obtained from Pharmacia (Piscataway NJ).

  • LacI/NLS (nuclear localization signal from SV40 large T-antigen) [ 25] was fused to a plant-optimized GFP sequence, mGFP5 [ 24], and designated mGFP5-LacI/NLS . mGFP5-LacI/NLS was inserted into a glucocorticoid-inducible expression vector pTA7002 [ 23] and the resulting vector designated pEL700.

  • The second was the random primer-labeled LacI/NLS . Membranes were prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C in ExpressHyb hybridization solution and washed at 60°C in washing solution.

  • The copy numbers of insertions in these lines were estimated with restriction digests of genomic DNA using Bgl II and Hind III in addition to Pst I, followed by Southern blot analysis using the LacI/NLS fragment as a probe (Figures 1, 2).


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