Words similar to krc
Example sentences for: krc
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Subsequently, sequence analysis identified KRC as a member of the ZAS family of proteins which share the ability to bind κB-like motifs [ 15 ] . DNA competition analysis showed that KRC fusion proteins containing the ZAS-C domain bind specifically to both the RSS and to the κB motif [ 14 28 ] . DNA footprinting analysis further showed that KRC/ZAS-C binds to specific nucleotides within the κB and the heptamer of the RSS [ 14 ] . In this study, using a PCR-based DNA-binding site-selection and amplification procedure, we demonstrated that both the N-terminal ZAS-N and the C-terminal ZAS-C domains are able to bind GT-rich DNA sequences, and confirmed that the RSS and κB motifs are the high-affinity targets of KRC.
Similarly, two closely migrating DNA-protein complexes were clearly seen for KRC/ZAS-C at rounds three through five (labeled C, Fig.
With respect to the heptamer (canonical sequence: CACAGTG), the CAC sequence bordering the recombination site is the most conserved segment of the sequence [ 32 ] , and mutation of these nucleotides has been found to decrease V(D)J joining in transfection assays using recombination substrates [ 40 ] . Because the sequence of the canonical RSS heptamer is palindrome, we speculate that the TGTG sequence may be sufficient for KRC/ZAS-C binding.
The fusion proteins KRC/ZAS-N and KRC/ZAS-C were produced in E. coli and purified by affinity chromatography as described previously [ 24 28 ] . The regions of KRC used to generate KRC/ZAS-N and KRC/ZAS-C are schematically shown in Fig.
Although RSS are evolutionarily conserved, the sequences of individual nonamer and heptamers vary [ 32 40 ] . To further determine the physiological significance of KRC's DNA binding, we compared the datasets with known endogenous RSSs of Ig and TCR loci.
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