Example sentences for: kpni

How can you use “kpni” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This plasmid was subsequently linearized with KpnI and used for electroporation.

  • To generate Rab24 fusions with Enhanced Green Fluorescent Protein (EGFP), cDNAs encoding full-length Rab24(wt) or Rab24(D123I), or the Rab24 C-terminal hypervariable domain (residues 166-203) were generated with flanking KpnI and BamHI sites and subcloned into pEGFP-C1 (Clontech Inc., Palo Alto, CA) so that the Rab inserts were in-frame with a 5' EGFP coding sequence.

  • A KpnI/XbaI digest liberated the CD2/hCARΔcyt transgene from the vector backbone and the transgene was subsequently microinjected into fertilized oocytes by the UAB ES/Transgenic facility.

  • This was inserted downstream of the SA in pPacSA digested with SalI, Klenow filled, and then cut with KpnI.

  • A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.


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