Example sentences for: kpni

How can you use “kpni” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A KpnI/XbaI digest liberated the CD2/hCARΔcyt transgene from the vector backbone and the transgene was subsequently microinjected into fertilized oocytes by the UAB ES/Transgenic facility.

  • A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.

  • Therefore, the mixture of theses two plasmids was digested with KpnI which linearized both plasmids, and the correct targeting vector was gel purified.

  • This was inserted downstream of the SA in pPacSA digested with SalI, Klenow filled, and then cut with KpnI.

  • This plasmid was subsequently linearized with KpnI and used for electroporation.


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