Words similar to kpn
Example sentences for: kpn
How can you use “kpn” in a sentence? Here are some example sentences to help you improve your vocabulary:
Oligonucleotide primers for the PCR reaction were based on the published sequence of murine Rab24 [ 24 ] . Kpn1 and BamH1 restriction sites were added to the 5' and 3' primers respectively, to facilitate cloning of the PCR product in frame with a 5' sequence cassette encoding a myc epitope (EQKLISEEDL), previously introduced into the pCMV5 expression vector [ 78 ] . The Rab24 cDNA was altered by overlap-extension PCR [ 79 ] , using appropriate mutator oligonucleotides and Pfu DNA polymerase (Stratagene Inc., La Jolla, CA), so as to introduce amino acid substitutions at D123 (D123I), T120 (T120A), S22 (S22N), S67 (S67Q), and a deletion of residues 127 through 130 (ΔEDRR).
A strain expressing HA-Ppt1p was made using the two-step gene replacement strategy [ 39 ] . First, the upstream sequence of PPT1 (-955 to -1) was amplified from yeast genomic DNA by PCR using a 5' primer containing a Kpn I restriction site (5'-gatcGGTACCattagcacggtgccttaacca-3') and a 3' primer containing a Xho I site and HA coding sequence (5'-gatcCTCGAGatcgataccgtcgagctagcgtagtctgggacgtcgtatgggtatcgactcatATTCGAGATATTTGATTATACCAAGGC-3').
This DNA was linearized with Kpn I and Sac I and then transformed into the diploid C. albicans strain BWP17 using the lithium acetate method.
4) The rpsL promoter was isolated from pCL8 as a Bam HI- Kpn I fragment and cloned into Bam HI- Kpn I digested pAWS2 upstream of the lacZ gene.
The amplified DNA fragment was cloned into pRS306 (New England Biolabs, Inc.) using Kpn I and Xho I restriction sites to generate pRS306-955HA.
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