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Example sentences for: kazusa

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To generate pGaL4-hCCR4, a fragment corresponding to the entire hCCR4 coding sequence (with the exception of the initiation codon) was obtained upon digestion of the KIAA1194 cDNA (accession number AB033020, gift from the Kazusa Research Institute, putative start codon -Met 19-positioned by homology with mCCR4) by Sph I and Msc I (nt 423 to 2172 in KIAA1194), and was inserted in-frame into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.
The program Codon Usage Tabulated from GenBank (CUTG) at http://www.kazusa.or.jp/codon/ [ 43 ] was used to calculate the codon usage of individual genes and genomes.
This organism is a widely used experimental model for the study of gene expression and plant-like oxygenic photosynthesis [ 7 ] . Synechocystis PCC6803 was predicted to contain 3,168 open reading frames (prior to May 2002) http://www.kazusa.or.jp/cyano/index.html [ 8 ] . Of these, over 50% (>1700 ORFs) have been characterized as hypothetical or having no similarity to known sequences.
The complete hCCR4 cDNA was obtained (KIAA1194 cDNA, Kazusa DNA Research Institute), and vectors were constructed to express hCCR4 fused to the DNA binding domain of the yeast GAL4 transcription factor (pGal4-hCCR4) (Fig.