Example sentences for: isotonic

How can you use “isotonic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Nuclear extracts were prepared by first lysing the cells for 10 minutes in 1 ml of isotonic buffer (50 mM KCl, 50 mM Tris-HCl, pH 7.9, 0.2% NP40, 0.5 mM EDTA and 1.5 mM MgCl2).

  • In order to allow for equilibration, pH i was measured 45 min after injection of 2.5 ml isotonic saline into the semipermeable balloon of the TRIP catheter.

  • Brush border membrane vesicles were prepared as described by deRooij [ 11 ] . Briefly, frozen ileal tissue fragments (20-25 mg) were thawed and homogenized in 100 μl of isotonic buffer (300 mM mannitol, 12 mM Tris-HCl, pH 7.1) using a Polytron homogenizer (Kinematica AG, Littau, Switzerland).

  • The initial response shows the current-voltage relationship recorded by two-electrode voltage clamp in an AQP1-expressing oocyte in isotonic 100 mM Na +saline (see Methods).

  • In Group I (control), 1 mL isotonic saline was given by intravenous infusion and 1 mL blood samples (to obtain basal vital measurements and biochemical parameters) were taken simultaneously at 0, 30, 60, 90, 120, 180, and 240 minutes.


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