Example sentences for: isothiocyanate

How can you use “isothiocyanate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, cells were stained with anti-human CD8− fluorescein isothiocyanate (Caltag Laboratories, Burlingame, California, United States) and CD19-CyChrome (BD Biosciences, Palo Alto, California, United States) Abs, and HLA-A*0201/peptide tetramer–phycoerythrin (PE).

  • BSA = bovine serum albumin; DMEM = Dulbecco's modified Eagle's medium; FACS = fluorescence-activated cell sorting; Fc = crystallizable fragment; FITC = fluorescein isothiocyanate; IFN = interferon; IL = interleukin; MHC = major histocompatibility complex; NF = nuclear factor; NO = nitric oxide; RANK = receptor-activator of NFκB; RANKL = receptor-activator of NFκB ligand; Th = T helper cells; TRAP = tartrate-resistant acid phosphatase.

  • The CD4 +CD25 +-expressing T cells were identified by staining spleen cells with fluorescein isothiocyanate (FITC)-labeled anti-CD4 and biotin-labeled anti-CD25 followed by CyChrome-labeled streptavidin (BD PharMingen, San Diego, CA, USA), and analyzed on a flow cytometer (Beckton Dickinson, San Jose, CA, USA).

  • The red fluorophore was excited using a TRITC (tetramethylrhodamine isothiocyanate) filter set (excitation 528-553 nm, dichroic 565LP, emission 600-660 nm).

  • Total RNA was purified by homogenization with guanidine isothiocyanate and phenol/chloroform extraction [ 28 ] . All procedures was performed at neutral pH.


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