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Example sentences for: isolates
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All sequencing reactions were performed in both forward and reverse directions, and all mutations were confirmed at least twice from independent PCR isolates.
The investigator isolates total and/or polyadenylated RNA from cells or tissues, generates the corresponding complementary DNA (cDNA), transcribes complementary RNA (cRNA) from the cDNA template, and then hybridizes the cRNA to the array [ 2].
In Bowie's failure to sell big in the States, James Miller isolates what I suspect is the real tragedy in his own critical life.
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
1 in two isolates: AR39 and AC43 (Figure 7).
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