Words similar to invertase
Example sentences for: invertase
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To pinpoint mutants that lacked secretion defects, we assayed each mutant from the collection for the ability to secrete the enzyme invertase in response to low glucose.
Assays of invertase activity were performed with 25 μl of each sample according to [ 70 71 ] . The activity of each mutant was normalized to the activity of the parental strain assayed at the same time.
However, yeast deletion mutants lacking all the p24 proteins grow similarly to wild type, although they show delays in translocation of a subset of cargo molecules such as invertase and Gas1p from the ER to the Golgi, and increased secretion of resident ER proteins [ 18].
Additionally, sec mutants with no apparent defect in invertase secretion ( npa1-3/sec23, npa1-7/sec23, and npa3-1/sec27 ) had both perturbations in ER structure and Nup localization.
Alternatively, these mutants may have defects along the pathway leading to transcriptional up-regulation of invertase ( SUC2 ) in response to low glucose [ 54 ] . Most importantly, 25% (25/96) of the mutants had at least 75% or greater of wild-type activity (Figure 8B).