Words similar to integrants

Example sentences for: integrants

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Here we constructed pct1Δ and pce1Δ plasmids containing 5' and 3' flanking genomic sequences in which the entire triphosphatase or guanylyltransferase coding sequence was deleted and replaced by the kanamycin resistance gene [ 17 ] . The pct1::kanMX and pce1::kanMX constructs were transformed separately into a diploid strain of S. pombe and chromosomal integrants containing one copy of the wild-type gene and one of pct1::kanMX or pce1::kanMX were selected on medium containing G418.
Second, because the integration site of the viral genome is variable, there can be local, host chromatin effects on exogenous gene expression and unpredictable silencing of viral integrants.
The transformations were performed using the lithium acetate method [ 18 ] . The integrants were selected at 30°C on YE plates containing 200 μg/ml G418.
Correct integrants were verified by PCR using primers that amplified a DNA fragment that spanned the recombination junction.
pombe his5 +PCR amplification/ transformation method was used [ 62 ] . Correct integrants were verified by PCR using primers that amplified a DNA fragment that spanned the recombination junction.