Words similar to incubating
Example sentences for: incubating
How can you use “incubating” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, TK-1 cells were suspended in culture medium and fluorescence labeled by incubating TK-1 cells at 2 × 10 6cells/ml with 0.02 mg fluorescein diacetate (FDA) (Sigma) at 37°C for 30 min.
After 6 hours of incubating macrophages with either alpha- or gamma-tocopherol (Figure 3A), the intracellular levels of gamma-tocopherol were at least six fold higher than the levels of alpha-tocopherol (p < 0.001).
The ternary complex (E-DNA-dNTP) formation was assessed by incubating the binary complexes of enzyme and dideoxy terminated template primer in the presence of next correct dNTP [ 25 ] . The binary complexes were formed by incubating 10-50 nM of the wild type enzyme or its mutant derivatives with 0.3 nM of 5'- 32P-labeled dideoxy terminated 33-mer/21-mer template-primer as described above.
To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC 18 ; Molecular Probes) as previously described [ 24 ] . Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging.
To verify that refolding of LA57-N 102LTat this temperature was not interfering with interpretation of the results, we further tested the SecB binding activity by incubating LA57-N 102LTin 8 M urea, then diluting it into the protection reaction, similar to the conditions used for proOmpA.
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