Words similar to incubating
Example sentences for: incubating
How can you use “incubating” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, TK-1 cells were suspended in Hank's Balanced salt solution (HBSS) and radiolabeled by incubating TK-1 cells at 2 × 10 7cells/ml with 30 μCi Na 51CrO 4 /ml (New England Nuclear, Natick, MA) at 37°C for 60 min.
After incubating for 1 hr on ice, the cells were washed, with a long period in PBS for the first wash as described above.
Cell envelopes were subjected to SDS PAGE and Coomassie blue staining using previously described methods [ 44 ] . Immunoblot assays were performed as described previously [ 45 ] . After incubating with the antibodies, blots were developed with the appropriate murine or rabbit peroxidase conjugated secondary antibody.
Accumulation of ADR was monitored using a standard procedure by incubating MCF7/ADR cells (5 × 10 5/ml) for 2 h at 37°C in the presence of ADR (10 μM) alone or in combination with NOM (20, 10 or 5 μM).
Specific bands were detected by incubating the membrane with horseradish peroxidase conjugated secondary antibody for 45 minutes at room temperature, then with ECL Western Blotting Detection Reagents (Amersham Life Science, Arlington Heights, IL) according to the manufacturer's protocol.