Example sentences for: incubating

How can you use “incubating” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.

  • The purified ButaIT was reduced and carboxymethylated by incubating in 6 M guanidine hydrochloride, 0.1 M Tris-HCl (pH 8.3), 1 mM EDTA and 20 mM dithiothreitol for 1 hr at 37°C.

  • The primers were 5'-labeled using γ- 32P-ATP and T4 polynucleotide kinase according to the standard protocol [ 37 ] . Polymerase reactions were carried out by incubating 2.5 nM template primer with 50 nM of the wild type HIV-1RT or its mutant derivative in a total reaction volume of 6 μL containing 25 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 100 μg/mL bovine serum albumin, 5 mM MgCl

  • First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.

  • cDNA was synthesized from each sample by incubating 1 ug RNA sample with 0.5 mM each dNTP, 0.25 μg/ml random hexamer (Boehringer Mannheim), 10 U RNase inhibitor, and 10 U OmniScript reverse transcriptase in OmniScript RT Buffer (Qiagen).

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