Example sentences for: incubating

How can you use “incubating” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The DNA on the microarray was then subjected to a UV-cross-linking at a dose of 150 mJ/cm 2. Prehybridization was performed by incubating the slide in a fresh mixture of 100 ml of 25% formamide, 5X SSC, 0.1% SDS, and 1% BSA for 45 min. at 42°C in a Coplin jar.

  • Briefly, TK-1 cells were suspended in Hank's Balanced salt solution (HBSS) and radiolabeled by incubating TK-1 cells at 2 × 10 7cells/ml with 30 μCi Na 51CrO 4 /ml (New England Nuclear, Natick, MA) at 37°C for 60 min.

  • Specific bands were detected by incubating the membrane with horseradish peroxidase conjugated secondary antibody for 45 minutes at room temperature, then with ECL Western Blotting Detection Reagents (Amersham Life Science, Arlington Heights, IL) according to the manufacturer's protocol.

  • [ 38 ] Briefly, radiolabeled phosphorylase a was synthesized by taking phosphorylase b (Sigma), at a concentration of 10 mg/ml, and incubating with 0.2 mg/ml phosphorylase kinase (Sigma) for 1.5 hours at 30°C in kinase reaction buffer (100 mM Tris-HCl; 100 mM sodium glycerol-1-phosphate, pH 8.2; 0.1 mM CaCl 2 , 10 mM magnesium acetate, and 0.2 mM [g- 32P]ATP {10 6cpm/nmol}).

  • Transposition was stopped by adding 1 μL of 1% SDS and incubating at 70°C for 10 min.

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