Example sentences for: incubating

How can you use “incubating” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After incubating 2 min at room temperature, the cells were washed once with 10 ml of RPMI medium + 10% fetal bovine serum (supplemented with L-glutamine and antibiotics).

  • Briefly, TK-1 cells were suspended in culture medium and fluorescence labeled by incubating TK-1 cells at 2 × 10 6cells/ml with 0.02 mg fluorescein diacetate (FDA) (Sigma) at 37°C for 30 min.

  • Sections were again washed 3× in PPS and color was evolved by incubating slides in the dark between 2-10 min in a 3,3'-diaminobenzidine (DAB) solution (Vector Laboratories Inc., Burlingame, CA).

  • The RNA was denatured prior to loading by incubating the RNA at 65°C for 10 min in a loading buffer comprising 1X MOPS, 50% formamide, 6.5% formaldehyde, 5% glycerol, 0.1 mM EDTA, 0.025% bromophenol blue, 0.025% xylene cyanol.

  • Reverse transcriptase activity in viral cores was assayed by incubating cores in endogenous polymerase buffer with 40 μM dATP, dCTP, dTTP and 2 μCi [α 32P]dGTP (Amersham) at 37°C for 90 min.


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