Example sentences for: incubated

How can you use “incubated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The filter was blocked with 3% BSA in TBS with 0.1% Tween 20 (T-TBS) for 30 min and incubated with the anti-phosphotyrosine antibodies at a dilution of 1:1000.

  • The resulting cleared supernatant was brought to 30% saturation with powdered ammonium sulfate and incubated with stirring for 30 min.

  • To determine the effect of the compounds on cell proliferation, after 24 h incubation, the cells were washed and further incubated in the absence of the compounds for 1 day, and then a trypan blue assay was done.

  • After several washes in PBS (room temperature), the sections were incubated with peroxidase labeled secondary antibody - goat anti-mouse IgG and IgM, pre-absorbed with human serum (Jackson Immunoresearch, West Grove, PA), diluted 1:50 and absorbed with rat kidney homogenate as described [ 39 ] . Tissue sections were processed further as described previously [ 40 ] , including hematoxylin counterstaining of the nuclei.

  • After preincubation with protein A-sepharose 4CL, the cell extract was incubated with specific mAb directly conjugated to sepharose 4B for 60 min, washed 3 times with the appropriate lysis buffer and eluted with Laemmli sample buffer.


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