Example sentences for: incubated

How can you use “incubated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 2 to 0.5 ng/nl) and were incubated for 2-5 days at 18°C in culture medium (ND96: 96 mM NaCl, 2 mM KCl, 1.8 mM CaCl 2 , 1 mM MgCl 2 , 5 mM Hepes, 2.5 mM pyruvic acid, 100 U/ml penicillin, and 100 μg/ml streptomycin, pH 7.6) to allow protein expression.

  • CV-1, BSC-40 and TK -143B cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and incubated at 37°C, 5% CO 2 . CV-1 cells were utilized for virus titer determinations and TK -143B cells were used in virus stock preparations.

  • The phage libraries (in NFM-PBS) are thawed and 100 μl of each library is added to the antigen-coated wells and the plates are incubated for 3 hours at room temperature.

  • Following 30 min incubation at 37°C, the cell suspension was diluted ∼ 10-fold with HBS, incubated for an additional 30 min, washed and resuspended in fresh HBS.

  • Up to 50 ng of CIP-treated template DNA were incubated for 20 minutes at 37°C in a 10 μL solution containing 20 U TdT (NEB), 0.2 M potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml BSA, 0.75 mM CoCl 2 , 4.6 μM dTTP and 0.4 μM ddCTP.


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