Example sentences for: incubated

How can you use “incubated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were permeabilized with 0.1% Triton X-100/TBS for 3 min, washed twice with TBS, blocked twice for 3 min with 0.1% bovine serum albumin in TBS, and incubated with rhodamine-conjugated phalloidin for 20 min.

  • For K i determinations, 100 μl of homogenate was incubated at 37°C for 2 hours with varying concentrations of inhibitor and [ 3H] granisetron (NEN, MA).

  • Membranes were blocked in 5% BLOT-QuickBlocker PBST buffer (plus 0.1% Tween-20), washed three times with PBST buffer (plus 0.1% Tween-20), incubated in primary antibody diluted in femto/TBST buffer in a tray for 90 min at room temperature (or overnight at 4°C), washed five times (as above), incubated in secondary antibody-HRP (horseradish peroxidase)-conjugate (goat anti-rabbit) as above, washed four times with femto/TBST buffer followed by transfer to detection buffer and detection reagent.

  • Iodoacetic acid was added to a final concentration of 50 mM and incubated for an additional hour at 37°C in the dark.

  • The sections were washed thrice with PBS and anti-digoxigenin-peroxidase conjugate was added on the section and incubated in a humidified chamber for 30 minutes at room temperature.


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