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Example sentences for: incognita
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The pectate lyase gene (MI00592) was identified in G. rostochiensis and H. glycines [ 17 ] and the exo-polygalacturonase (MI00252) was identified in our M. incognita data [ 21 22 ] . Because of the obvious role of nematode genes that allow endogenous production of cell-wall degrading enzymes in attacking a plant host, it has been hypothesized that their acquisition by HGT may have been key steps in the evolution of plant-parasitic nematodes from ancestral free-living forms [ 3 ] . In that model, an intermediate, symbiotic association of a soil-dwelling (but free-living) nematode with a soil bacterium possessing these enzymes is postulated before the HGT event.
The GO categorization scheme (go_200205-assocdb.sql) of classification by biological process, cellular component, and molecular function was used to classify clusters based on the existing mappings of InterPro domains to the GO hierarchy [ 36 ] . Mappings were stored in a local MySQL database and displayed using the AmiGO browser (16 May 2002) [ 100 ] ( M. incognita mappings at [ 101 ] ).
Following isolation from G. rostochiensis and H. glycines [ 5 ] , cellulases have been identified in M. incognita [ 6 ] , G. tabacum [ 68 ] , H. schachtii [ 69 ] , and P. penetrans [ 70 ] . Additional prokaryotic-like sequences identified in plant parasitic nematodes include other cell-wall-degrading enzymes such as xylanase [ 7 ] , pectate lyase [ 8 71 ] and polygalacturonase [ 72 ] , and evidence is accumulating that these sequences have been acquired by horizontal gene transfer [ 11 ] . The known Meloidogyne cellulase (MI00483.cl), potentially novel cellulases (MI00537.cl, MI01196.cl, MI01381.cl, MI01842.cl), and pectate lyase (MI00592.cl, MI00520.cl) were represented in the M. incognita EST clusters.
Sequences were obtained from the Parasitic Nematode Sequencing Project (PNSP) [ 30 ] including clustered Meloidogyne ESTs built with the NemaGene approach [ 28 ] . We analyzed 1,799 M. incognita (WMi) sequences and 3,119 M. javanica (WMj) sequences from these PNSP clusters.
The only previous analysis of root knot nematodes ESTs [ 29 ] used 914 ESTs from M. incognita L2 without clustering and with non-automated assignment of genes to categories.
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