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Example sentences for: incognita
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Following isolation from G. rostochiensis and H. glycines [ 5 ] , cellulases have been identified in M. incognita [ 6 ] , G. tabacum [ 68 ] , H. schachtii [ 69 ] , and P. penetrans [ 70 ] . Additional prokaryotic-like sequences identified in plant parasitic nematodes include other cell-wall-degrading enzymes such as xylanase [ 7 ] , pectate lyase [ 8 71 ] and polygalacturonase [ 72 ] , and evidence is accumulating that these sequences have been acquired by horizontal gene transfer [ 11 ] . The known Meloidogyne cellulase (MI00483.cl), potentially novel cellulases (MI00537.cl, MI01196.cl, MI01381.cl, MI01842.cl), and pectate lyase (MI00592.cl, MI00520.cl) were represented in the M. incognita EST clusters.
The GO categorization scheme (go_200205-assocdb.sql) of classification by biological process, cellular component, and molecular function was used to classify clusters based on the existing mappings of InterPro domains to the GO hierarchy [ 36 ] . Mappings were stored in a local MySQL database and displayed using the AmiGO browser (16 May 2002) [ 100 ] ( M. incognita mappings at [ 101 ] ).
To identify cases where M. incognita and C. elegans share homologous genes which have been surveyed in C. elegans for knockout phenotype using RNAi, a list of all 7,212 available C. elegans RNAi experiments (5 May 2002) from WormBase [ 56 ] was compared to the list of all M. incognita clusters with significant homology matches to the C. elegans Wormpep v.54 protein database.
Of 1,625 clusters, 1,280 (79%) have homologies beyond M. incognita , 693 (43%) align to InterPro domains, and 475 (29%) map to the GO hierarchy.
NemaGene Meloidogyne incognita v 2.0 represents our second complete attempt at generating clusters for this species and is used as the basis for all subsequent analysis in this manuscript.