Example sentences for: imidazole

How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1 % Triton X-100, 80 mM KCl, 20 mM imidazole, 2 mM MgCl 2 , 2 mM EGTA, pH 7.8] for 5 min at 4°C.

  • Following incubation at room temperature for 10 min, the bound proteins were eluted with buffer A plus 1 M imidazole, and then analyzed by SDS-PAGE and Western blot, as and where mentioned.

  • The heterodimeric RT was then eluted from the column with elution buffer (20 mM Tris HCl pH 7.8, 500 mM NaCl and 250 mM imidazole).

  • Buffer B (6 M urea, 0.1 M NaH 2 PO 4 , 10 mm Tris-l, 0.1 M KCl), used for cell lysis and protein elution, was prepared with the addition of imidazole (final concentration 25 mm) and urea (final concentration approximately 8 M).

  • Purified protein was eluted from the column with buffer B containing 100-250 mm imidazole.


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