Example sentences for: imidazole

How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.

  • Buffer B (6 M urea, 0.1 M NaH 2 PO 4 , 10 mm Tris-l, 0.1 M KCl), used for cell lysis and protein elution, was prepared with the addition of imidazole (final concentration 25 mm) and urea (final concentration approximately 8 M).

  • Pooled Macroprep Q fractions containing GFP-BLM were loaded onto a 5 ml HiTrap metal chelate column (Amersham Pharmacia) charged with NiS0 4 equilibrated in Buffer B. The metal chelate resin was washed with successive 10 column volumes of Buffer B + 10mm imidazole, Buffer B + 50 mM imidazole, and Buffer B + l00 mM imidazole.

  • The pathogenic mutation of arginine to histidine at this position represents a moderately significant divergence in sequence, substituting an imidazole group for a guanidino group.

  • Purified protein was eluted from the column with buffer B containing 100-250 mm imidazole.


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