Example sentences for: imidazole

How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Purified protein was eluted from the column with buffer B containing 100-250 mm imidazole.

  • Following incubation at room temperature for 10 min, the bound proteins were eluted with buffer A plus 1 M imidazole, and then analyzed by SDS-PAGE and Western blot, as and where mentioned.

  • Likewise, subfamily Iβ partitions into narrow-specificity imidazole acetol phosphate (IAP) aminotransferases and broad-specificity IAP aminotransferases that utilize aromatic amino/keto acids.

  • Pooled Macroprep Q fractions containing GFP-BLM were loaded onto a 5 ml HiTrap metal chelate column (Amersham Pharmacia) charged with NiS0 4 equilibrated in Buffer B. The metal chelate resin was washed with successive 10 column volumes of Buffer B + 10mm imidazole, Buffer B + 50 mM imidazole, and Buffer B + l00 mM imidazole.

  • 15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.


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