Example sentences for: imidazole

How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.

  • Following incubation at room temperature for 10 min, the bound proteins were eluted with buffer A plus 1 M imidazole, and then analyzed by SDS-PAGE and Western blot, as and where mentioned.

  • Highly purified GFP-BLM eluted in Buffer B + 200 mM imidazole and was stored at -70°C.

  • Purified protein was eluted from the column with buffer B containing 100-250 mm imidazole.

  • The heterodimeric RT was then eluted from the column with elution buffer (20 mM Tris HCl pH 7.8, 500 mM NaCl and 250 mM imidazole).


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