Words similar to imidazole
Example sentences for: imidazole
How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:
1 % Triton X-100, 80 mM KCl, 20 mM imidazole, 2 mM MgCl 2 , 2 mM EGTA, pH 7.8] for 5 min at 4°C.
Following incubation at room temperature for 10 min, the bound proteins were eluted with buffer A plus 1 M imidazole, and then analyzed by SDS-PAGE and Western blot, as and where mentioned.
The heterodimeric RT was then eluted from the column with elution buffer (20 mM Tris HCl pH 7.8, 500 mM NaCl and 250 mM imidazole).
Buffer B (6 M urea, 0.1 M NaH 2 PO 4 , 10 mm Tris-l, 0.1 M KCl), used for cell lysis and protein elution, was prepared with the addition of imidazole (final concentration 25 mm) and urea (final concentration approximately 8 M).
Purified protein was eluted from the column with buffer B containing 100-250 mm imidazole.