Words similar to imidazole
Example sentences for: imidazole
How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:
1 % Triton X-100, 80 mM KCl, 20 mM imidazole, 2 mM MgCl 2 , 2 mM EGTA, pH 7.8] for 5 min at 4°C.
15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.
Highly purified GFP-BLM eluted in Buffer B + 200 mM imidazole and was stored at -70°C.
Following incubation at room temperature for 10 min, the bound proteins were eluted with buffer A plus 1 M imidazole, and then analyzed by SDS-PAGE and Western blot, as and where mentioned.
Buffer B (6 M urea, 0.1 M NaH 2 PO 4 , 10 mm Tris-l, 0.1 M KCl), used for cell lysis and protein elution, was prepared with the addition of imidazole (final concentration 25 mm) and urea (final concentration approximately 8 M).
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