Example sentences for: imidazole

How can you use “imidazole” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1 % Triton X-100, 80 mM KCl, 20 mM imidazole, 2 mM MgCl 2 , 2 mM EGTA, pH 7.8] for 5 min at 4°C.

  • Buffer B (6 M urea, 0.1 M NaH 2 PO 4 , 10 mm Tris-l, 0.1 M KCl), used for cell lysis and protein elution, was prepared with the addition of imidazole (final concentration 25 mm) and urea (final concentration approximately 8 M).

  • Likewise, subfamily Iβ partitions into narrow-specificity imidazole acetol phosphate (IAP) aminotransferases and broad-specificity IAP aminotransferases that utilize aromatic amino/keto acids.

  • Pooled Macroprep Q fractions containing GFP-BLM were loaded onto a 5 ml HiTrap metal chelate column (Amersham Pharmacia) charged with NiS0 4 equilibrated in Buffer B. The metal chelate resin was washed with successive 10 column volumes of Buffer B + 10mm imidazole, Buffer B + 50 mM imidazole, and Buffer B + l00 mM imidazole.

  • Highly purified GFP-BLM eluted in Buffer B + 200 mM imidazole and was stored at -70°C.

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