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Example sentences for: iiib
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To measure the virucidal activity of CAP/sCD4 combinations, treated and untreated purified HIV-1 IIIB (3.
Treatment of HIV-1 IIIB and BaL with CAP did not decrease the level of virus-associated gp120 in comparison with control virus preparations (Table 3).
In repeated experiments with HIV-1 IIIB, soluble and micronized CAP, cellulose sulfate, poly(naphthalene sulfonate), poly(styrene-4-sulfonate) and BufferGel, respectively, caused a ≥ 140-fold reduction in infectivity, while dextran sulfate, carrageenan and Carbomer 974P (pH 7), respectively, were less effective (4- to 25-fold reduction in infectivity).
In summary, the contact of HIV-1 IIIB and HIV-1 BaL, representatives of X4 and R5 viruses respectively, with micronized CAP, resulted in a complex sequence of events involving virus adsorption, exposure to low pH, induction of gp41 six-helix bundles, and stripping off of viral envelopes and virus disintegration, ultimately resulting in loss of infectivity.
The percentage of residual infectivity after CAP treatment was calculated from calibration curves relating absorbance (corresponding to formazan for HIV-1 IIIB and p24 antigen for HIV-1 BaL, respectively) to virus dilutions of untreated viruses.