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Example sentences for: igfbp
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Also of interest are genes that are upregulated in both the cancers, including IGFBP3 (insulin-like growth factor binding protein 3), which, interestingly, is thought to have proapoptotic activities [ 14 ] , and GSN (gelsolin), which has a role in cellular motility and acts as both a regulator and effector of apoptosis [ 15 ] . Further xenograft studies of the type presented here could examine the responses of the host tissue to the tumor (using a mouse microarray chip), as well as temporal changes in gene expression within the developing tumor.
To confirm whether the new information by this approach is reliable, we randomly picked up two differentially expressed genes, IGFBP2 and integrin beta 4, that were identified by the amplification protocol but not by the regular protocol for confirmation using western blotting, northern blotting and immunohistochemistry assays.
et al . found significantly different baseline expression levels of insulin-like growth factor (IGF) and insulin-like growth factor binding protein (IGFBP) between normal and AIS-derived genital skin fibroblasts, and could not detect changes in transcript levels in response to androgen treatment [ 29 ] . In normal genital fibroblasts, androgen-responsive reporter genes can only be activated by expression of co-transfected AR in the presence of ligand [ 30 ] . Therefore, endogenous AR expression itself may be insufficient in genital skin fibroblasts to elicit a transcriptional response.
For western blotting, 40 μg of total cellular protein was run on a SDS-PAGE gel, transferred to a nylon membrane, and incubated with IGFBP2 antibody (Santa Cruz Biotechnology, Inc.) following the procedure as described [ 11 ] .
The presence of S100, GFAP, and IGFBP2 antigens was detected by their antibodies following standard immunohistochemistry procedures [ 11 ] .
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