Example sentences for: igepal

How can you use “igepal” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Subcellular fractionation of RNA into cytoskeleton-associated and cytosolic fractions was performed as previously described [ 15 34 ] . In brief, HL-60 cells were harvested by centrifugation and resuspension in buffer A (10 mM Tris pH 7.6, 0.25 M sucrose, 25 mM KCl, 5 mM MgCl 2 ) with 0.1% Igepal nonionic detergent (Sigma).

  • TPA-treated cells become adherent and were harvested at 40 hr by scraping in buffer A, pelleting the cells, and resuspending in buffer A plus 0.1% Igepal.

  • Cell lysates were obtained by rinsing 12-day old neuronal cell cultures with ice-cold phosphate-buffered saline prior to trituration in ice-cold lysis buffer (PBS supplemented with 1% Igepal, 0.5% sodium deoxycholate, 0.1% SDS, 100 μg/ml PMSF and 300 μg/ml aprotinin).

  • Frozen lung tissue was homogenized in ice cold 750 μl radioimmunoprecipitation assay (RIPA) buffer (1×PBS, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors (30 μl/ml RIPA aprotinin, 10 μl/ml RIPA 100 mmol/l sodium orthovanadate).


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