Words similar to ice-cold

Example sentences for: ice-cold

How can you use “ice-cold” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were labeled with 1 mCi Na 125I and lactoperoxidase-H 2 O 2 , as previously described [ 27 ] . After labeling the cell pellet was washed with ice-cold PBS + NaN 3 , then lysed in 2 volumes of OG Lysis Buffer (200 mM octylglucoside, 1 mM CaCl 2 , 1 mM MgCl 2 , TBS, PMSF) at 4°C.
5μl of ice-cold protein binding buffer (PBB) along with 10μl of GST-bead slurry corresponding to 3μg of fusion protein, 1μl of in vitro translated protein and 1.5μl of ligand or vehicle and/or peptides or vehicle.
The growing, adherent cells were rinsed in phosphate-buffered saline (PBS), fixed in ice-cold 4% paraformaldehyde for 20 min, and then washed in PBS.
The cells were then washed twice with ice-cold HBSS, spun at 250 g for 5 min to remove unincorporated fluorescence and suspended in HBSS.
The reaction was stopped by adding 1 ml of ice-cold binding buffer followed by rapid filtration on Inotech IH-201-A21 glass fiber filters employing an Inotech 96 well filtration manifold (Inotech Biosystems, Lansing, MI).