Words similar to ice-cold

Example sentences for: ice-cold

How can you use “ice-cold” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were washed five times with ice-cold PBS containing 0.05% saponin and sonicated in 100 mM sodium acetate (pH 4.6).
Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.
The cells were then washed twice with ice-cold HBSS, spun at 250 g for 5 min to remove unincorporated fluorescence and suspended in HBSS.
Cellular fractionation was performed as described in earlier reports [ 122 123 124 125 ] . Cells were scraped into 100 μl of ice-cold buffer A [10 mM Hepes (pH 7.9); 1.5 mM MgCl 2 ; 10 mM KCl; 0.5 mM DTT; 0.05% NP-40].
The reaction was stopped by adding 1 ml of ice-cold binding buffer followed by rapid filtration on Inotech IH-201-A21 glass fiber filters employing an Inotech 96 well filtration manifold (Inotech Biosystems, Lansing, MI).