Words similar to ice-cold

Example sentences for: ice-cold

How can you use “ice-cold” in a sentence? Here are some example sentences to help you improve your vocabulary:

The FNA material was immediately resuspended in ice-cold Iscove's medium without serum supplementation, and subsequently washed in PBS before RNA isolation.
Cells were labeled with 1 mCi Na 125I and lactoperoxidase-H 2 O 2 , as previously described [ 27 ] . After labeling the cell pellet was washed with ice-cold PBS + NaN 3 , then lysed in 2 volumes of OG Lysis Buffer (200 mM octylglucoside, 1 mM CaCl 2 , 1 mM MgCl 2 , TBS, PMSF) at 4°C.
Ice-cold yersiniae were resuspended in ice-cold PBS and passed through a French pressure cell at 20,000 psi.
For preparation of protein lysates from placental tissue and ovarian cancer for western blot analysis, including control Cdk2 and normal rabbit immunoglobulin immunoprecipitates (IP), 150 cryostat tissue sections (7μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
At regular intervals, the slides were rinsed in PBS, fixed in ice-cold 4% paraformaldehyde for 20 min, washed in PBS, stained with anti-BMPR antibodies, and visualized by the ABC immunoperoxidase method described above.