Words similar to ice-cold

Example sentences for: ice-cold

How can you use “ice-cold” in a sentence? Here are some example sentences to help you improve your vocabulary:

The nuclei prepared above were suspended in 10 ml of ice-cold TP buffer (10 mM Tris/HCl, pH 8.0, 10 mM Na 2 HPO 4 , 1 mM PMSF) containing 0.3 mg/ml heparin and 36 u/ml of DNase I. The suspension was gently stirred on a magnetic stirrer for 60 minutes at 4°C and 15 minutes at room temperature.
Cells were collected in microfuge tubes containing 1 ml of ice-cold 90% IMDM with 10% FBS.
The filtrate was washed twice with PBS, fixed in 70% ice-cold ethanol, and stored at 4°C.
The reaction was stopped by adding 1 ml of ice-cold binding buffer followed by rapid filtration on Inotech IH-201-A21 glass fiber filters employing an Inotech 96 well filtration manifold (Inotech Biosystems, Lansing, MI).
Rabbit polyclonal antibody against RSV-P protein has been described [ 21 ] . Indirect immunofluorescence and nuclear staining with DAPI was performed essentially as described previously [ 33 ] . A549 cells in monolayer, grown on cover slips, were washed in PBS and fixed in ice-cold 10% trichloracetic acid for 15 min, followed by successive washes in cold 70%, 90% and absolute ethanol for 3 min each.