Words similar to ice-cold

Example sentences for: ice-cold

How can you use “ice-cold” in a sentence? Here are some example sentences to help you improve your vocabulary:

Following the incubation, the cells were washed 5 times with ice-cold PBS and subjected to enzyme activity assay as described above.
5μl of ice-cold protein binding buffer (PBB) along with 10μl of GST-bead slurry corresponding to 3μg of fusion protein, 1μl of in vitro translated protein and 1.5μl of ligand or vehicle and/or peptides or vehicle.
The cell pellets were suspended in 150-300 μl ice-cold RIPA lysis buffer (1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS in PBS, 0.5 mM Phenylmethylsulfonyl fluoride (PMSF), 0.02 mg/ml Aprotinin, 1 mM Na 3 VO 4 ) and incubated for 30 min on ice.
The cells were then washed twice with ice-cold HBSS, spun at 250 g for 8 min to remove unincorporated radioactivity and resuspended in HBSS.
To extract RNA from tissue, 100 mg of rat lung was ground in 6 ml of ice-cold resuspension buffer.