Example sentences for: ice-cold

How can you use “ice-cold” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The following manipulations were performed either with ice-cold reagents or at 4°C unless noted.

  • Briefly, flies were attached with head and thorax to a small plastic stick and submerged into ice-cold Drosophila saline.

  • Smooth muscle preparations were frozen by immersion in ice-cold acetone containing 5 mM NaF (-80°C).

  • The cells were then washed twice with ice-cold HBSS, spun at 250 g for 8 min to remove unincorporated radioactivity and resuspended in HBSS.

  • To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .


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