Example sentences for: ice

How can you use “ice” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Subsequently the wells were washed 10× with ice cold PBS or 1:50 anti-p24 mAb in Ch-PBS for HIV-1 BaL to minimize the contribution of p24 antigen not associated with virus particles to absorbance readings corresponding to p24 antigen released from detergent treated virus.

  • Plates were placed on ice and incubation buffer aspirated.

  • This suspension was cleared form debris and diluted with five volumes of ice cold water and MgCl 2 was added to a final concentration of 10 mM.

  • Brains for Northern blot analysis were quickly removed and the hypothalamus was dissected out, frozen on dry ice and stored at -70°C until use as described previously [Makimura, 2000 #5346; [Mizuno, 1999 #5302].

  • Sections were fixed for 1 minute in ice cold methanol; blocked for 45 minutes in blocking buffer (15% horse serum, 0.025% Triton, 1x PBS); incubated overnight at 4°C with anti-sarcospan (1:750) and anti-spectrin (1:100) antibodies in block buffer; washed 3 × for 15 minutes in wash buffer (0.


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