Words similar to hypoxanthine
Example sentences for: hypoxanthine
How can you use “hypoxanthine” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
However, the variants were no longer responsive to theophylline, nor were they responsive to a variety of other analogues, including caffeine, 1-methylxanthine, 7-methylxanthine, 1,3-dimethyl urilic acid, hypoxanthine, xanthine, and theobromine (Figure 6b).
P. falciparum parasite cells (W2 strain) were cultured as described [ 54] with slight modifications: 2% suspension of purified human red blood cells in RPMI1640 media supplemented with 0.25% AlbumaxI (GIBCO/Invitrogen, San Diego, CA), 2 g/l sodium bicarbonate, 0.1 mM hypoxanthine, 25 mM HEPES pH 7.4, and 50 μg/I gentamycin.
Fold induction was obtained by using the ΔΔC t method in which all samples are first normalized to the level of mouse hypoxanthine phosphoribosyltransferase (HPRT) in each sample.
Loss of PfPP1 resulted in concomitant inhibition of parasite growth as evidenced by the drastic reduction in 3H-hypoxanthine incorporation.