Words similar to hypoxanthine
Example sentences for: hypoxanthine
How can you use “hypoxanthine” in a sentence? Here are some example sentences to help you improve your vocabulary:
Fold induction was obtained by using the ΔΔC t method in which all samples are first normalized to the level of mouse hypoxanthine phosphoribosyltransferase (HPRT) in each sample.
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
P. falciparum parasite cells (W2 strain) were cultured as described [ 54] with slight modifications: 2% suspension of purified human red blood cells in RPMI1640 media supplemented with 0.25% AlbumaxI (GIBCO/Invitrogen, San Diego, CA), 2 g/l sodium bicarbonate, 0.1 mM hypoxanthine, 25 mM HEPES pH 7.4, and 50 μg/I gentamycin.
However, the variants were no longer responsive to theophylline, nor were they responsive to a variety of other analogues, including caffeine, 1-methylxanthine, 7-methylxanthine, 1,3-dimethyl urilic acid, hypoxanthine, xanthine, and theobromine (Figure 6b).
Loss of PfPP1 resulted in concomitant inhibition of parasite growth as evidenced by the drastic reduction in 3H-hypoxanthine incorporation.