Example sentences for: hpop

How can you use “hpop” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The pVP16-hCAF1 and pVP16-hPOP2 constructs were obtained upon insertion of the hCAF1 and hPOP2 coding regions into the pSG5FNV vector, in-frame with the VP16 transactivation domain coding sequence, as previously described [ 17 25 ] .

  • Third, the ability of yCCR4 to bind mCAF1 and hPOP2 is conserved for the mammalian protein, since we could demonstrate, using two-hybrid assays and far-Western blot analyses, an in vivo and in vitro interaction between hCCR4 and hCAF1 or hPOP2/CALIF.

  • 4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.

  • Hence, the present identification of the human ortholog of yCCR4 and the demonstration that it binds to hPOP2 now provide an additionnal piece of the puzzle, strongly suggesting the existence of a CCR4-NOT complex in humans.

  • Here we report the identification of nineteen yCCR4-like proteins in eukaryotes and show, by phylogenetic and genomic analyses, that they are grouped into four distinct families, one of which contains the yCCR4 orthologs : these orthologs, in animals, have conserved the yCCR4 leucine-rich repeat, and we show, using two-hybrid assays and far-Western experiments, that the human protein binds to the human yPOP2 homologs, i.e. hCAF1 and hPOP2, in a LRR-dependent manner.


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