Words similar to homeologs
Example sentences for: homeologs
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However, the E -value for each BLAST hit was quite different between TBLASTX and BLASTN, suggesting that an arbitrary threshold E -value is critical to determine acceptance of BLAST matches between evolutionarily distant nucleotide sequences and to place the homeologs on Arabidopsis chromosomes.
Given that several genes exist between the homeologs of the two closest flanking MESTs, TC85079 (EFP) and TC98260 (GRP), and neither a deletion nor local rearrangement of the Dgt counterpart has occurred in the MSRs, the Dgt counterpart could be one of the annotated genes between the homeologs of the two closest flanking MESTs.
The second microsyntenic region (MSR2) spanned two Arabidopsis BAC clones, F11l11 (AL079347) and M4E13 (AL022023), and was also located on chromosome 4, but only yielded homeologs for tomato markers TG269 and CT190 ( E -values of 1e -3and 8e -3, respectively).
Moreover, two recent comparative sequence analyses using monocots versus Arabidopsis [ 29] and dicots versus Arabidopsis [ 11] clearly showed that exon sizes are well conserved in homeologs even between monocots and dicots, whereas intron length varied in rice versus barley and tomato versus Arabidopsis . In BLAST searches using either the processed nucleotide sequence or the predicted protein sequence of an Arabidopsis gene, the score of each BLAST match was clearly higher and the E -value was significantly lower when compared to the use of either non-processed nucleotide sequence or intergenic nucleotide sequence.
Because Arabidopsis MSR1 contained homeologs in common with three RFLP markers in the dgt region of tomato, we used it as our main target region to search for corresponding tomato homeologs.