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Example sentences for: hnrnp
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Primers complementary to adjacent hnRNP A2/B1 exon sequences (PR1 or PR2) and a neo-specific primer (NeoB) were used to amplify transcripts extending from the hnRNP A2/B1 gene into the provirus (Figure 5A).
Continued expression of hnRNP A2/B1 transcripts suggests that use of the viral poly(A) sites is less efficient than removal of the intron in which the provirus resides.
adjacent to the 1B4 provirus were isolated by inverse polymerase chain reaction (PCR) as reported elsewhere [ 29 ] . This flanking sequence was used as a probe to genotype mutant mice and cells by Southern blot hybridization and was also used to isolate cDNA clones encoding the murine hnRNP A2/B1 protein from a PCC3 embryonal carcinoma cell cDNA library.
As shown in Figure 5D, all of these transcripts spliced from the proximal 5' splice site in the hnRNP A2/B1 gene to a cryptic 3' splice site located in the Neo gene (Figure 5D).
Other hnRNP A2/B1 transcripts splice normally, removing the provirus along with other intron sequences.