Words similar to hmyoc

Example sentences for: hmyoc

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Next we created a human myocilin expression construct where the N-terminal 14aa were deleted (pcDNA3.hMYOC.ΔN14) forcing translation to begin at the second methionine codon (Fig.
PCR and processing were as described above with the exception that the 5' human MYOC deletion products were cloned into a HindIII/Bsu36I digested pcDNA3.hMYOC wild-type vector.
Transfection of GTM66 cells with pcDNA3.hMYOC.ΔN32 resulted in intracellular but not extracellular myocilin distribution (Fig.
Transfected plasmid DNA included wild-type human MYOC (pcDNA3.hMYOC; [ 12 ] ), wild-type rabbit MYOC (pcDNA3.RbMYOC.ex1-3), N-terminal 14 amino acid deleted human MYOC (pcDNA3.hMYOC.ΔN14), N-terminal 32 amino acid deleted human MYOC (pcDNA3.hMYOC.ΔN32), or N57S human MYOC (pcDNA3.hMYOC.N57S).
A signal peptide cleavage site was predicted to exist between rabbit aa 18 and 19 (human aa 32 and 33) [ 33 ] . We directly tested the importance of the signal peptide in myocilin secretion by replacing the first 32 aa of human myocilin with a single methionine (pcDNA3.hMYOC.ΔN32).