Words similar to his-tagged

Example sentences for: his-tagged

How can you use “his-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:

His-tagged P protein was expressed by IPTG-mediated induction of a pET-15b-RSV P (Long) clone in E. coli BL21(DE3).
For each set of heterodimers, 260 μg of p51 was mixed with 660 μg of p66 in the buffer containing 50 mM Tris HCl, pH 7.8, 60 mM KCl and 5 mM MgCl 2 . The rationale for using a 1:3 ratio of p51 to p66 was to saturate the His-tagged p51 with the non-tagged p66, ensuring heterodimer formation and eliminating excess p66 during IMAC purification.
As expected, the native Pf protein was about 2 kDa smaller than the His-tagged recombinant (compare the native band in lanes 2, 5, and 6 with the recombinant in lanes 4 and 8).
Recombinant His-tagged P protein was immobilized on a Ni-NTA column and purified recombinant human profilin was passed through it.
Purification of His-tagged protein