Words similar to his-tagged

Example sentences for: his-tagged

How can you use “his-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:

His-tagged P protein was expressed by IPTG-mediated induction of a pET-15b-RSV P (Long) clone in E. coli BL21(DE3).
Recombinant His-tagged P protein was immobilized on a Ni-NTA column and purified recombinant human profilin was passed through it.
The imidazole-eluted His-tagged PfPP1 was dialyzed against 50 mM Tris-Cl (pH 7.5), 100 mM NaCl, 25% glycerol, 1 mM DTT (buffer A), and stored in small portions at -80°C.
Purification of His-tagged protein
For each set of heterodimers, 260 μg of p51 was mixed with 660 μg of p66 in the buffer containing 50 mM Tris HCl, pH 7.8, 60 mM KCl and 5 mM MgCl 2 . The rationale for using a 1:3 ratio of p51 to p66 was to saturate the His-tagged p51 with the non-tagged p66, ensuring heterodimer formation and eliminating excess p66 during IMAC purification.