Words similar to his-
Example sentences for: his-
How can you use “his-” in a sentence? Here are some example sentences to help you improve your vocabulary:
6His-Myc, or pRep4.HA, and sequenced.
In contrast, it appears that for the doxycycline-induced cells (third lane), there is more 6His-HA-PP1α RNA associated with polysomes than endogenous PP1α.
The doxycycline-independent increase in 6His-HA-PP1α is attributed to background expression when using this system [ 25 ] . As predicted for a protein under the control of the doxycycline-inducible promoter, the abundance of 6His-HA-PP1α decreases in the absence of the inducer over time (Figure 2C).
Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).
To further investigate whether COP1 itself undergoes ubiquitination, a His-tagged ubiquitin expression construct (His-Ub) was co-transfected into the 293 cells with FLAG-COP1, followed by His purification under denaturing condition (in 8 M Urea).
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