Example sentences for: his-

How can you use “his-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 6His-Myc, or pRep4.HA, and sequenced.

  • In contrast, it appears that for the doxycycline-induced cells (third lane), there is more 6His-HA-PP1α RNA associated with polysomes than endogenous PP1α.

  • The doxycycline-independent increase in 6His-HA-PP1α is attributed to background expression when using this system [ 25 ] . As predicted for a protein under the control of the doxycycline-inducible promoter, the abundance of 6His-HA-PP1α decreases in the absence of the inducer over time (Figure 2C).

  • Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).

  • To further investigate whether COP1 itself undergoes ubiquitination, a His-tagged ubiquitin expression construct (His-Ub) was co-transfected into the 293 cells with FLAG-COP1, followed by His purification under denaturing condition (in 8 M Urea).


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