Example sentences for: his-

How can you use “his-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • If mRNA association with ribosomes is truly a measure of translation efficiency, one conclusion from these data is that in doxycycline-induced cells, 6His-HA-PP1α RNA is more efficiently translated than endogenous PP1α.

  • As such, we have chosen the 24 hr induction time for all subsequent experiments to ensure peak abundance of the induced 6His-HA-PP1α.

  • The doxycycline-independent increase in 6His-HA-PP1α is attributed to background expression when using this system [ 25 ] . As predicted for a protein under the control of the doxycycline-inducible promoter, the abundance of 6His-HA-PP1α decreases in the absence of the inducer over time (Figure 2C).

  • To further investigate whether COP1 itself undergoes ubiquitination, a His-tagged ubiquitin expression construct (His-Ub) was co-transfected into the 293 cells with FLAG-COP1, followed by His purification under denaturing condition (in 8 M Urea).

  • Under this condition, only those proteins covalently linked to His-Ub would be co-purified.


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