Words similar to his-
Example sentences for: his-
How can you use “his-” in a sentence? Here are some example sentences to help you improve your vocabulary:
A cocktail was added that contained 8 μM ubiquitin, ATP regenerating system (2 mM HEPES at pH 7.4, 1 mM ATP, 30 mM creatine phosphate, 1 mM magnesium acetate, 0.15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1mM DTT), 500 nM bacterially expressed 6 × His-UBCs, 100 nM 6 × His-tagged human E1, 0.5 μM ubiquitin aldehyde in a volume of 15 ul.
Indeed, a time course of induction followed by western blotting of whole cell lysates using antibody to PP1α revealed diminution of the endogenous PP1α signal after 4 hr of induction, while the 6His-HA-PP1α signal increased (Figure 8).
Localization of this protein to the nucleus also mirrors that of endogenous PP1α in vivo . When using this system to forcibly overexpress 6His-HA-PP1α, there is a concomitant decrease in endogenous PP1α levels, suggesting the existence of an autoregulatory mechanism by which PP1α protein levels remain constant.
Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).
If mRNA association with ribosomes is truly a measure of translation efficiency, one conclusion from these data is that in doxycycline-induced cells, 6His-HA-PP1α RNA is more efficiently translated than endogenous PP1α.
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