Example sentences for: his-

How can you use “his-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A cocktail was added that contained 8 μM ubiquitin, ATP regenerating system (2 mM HEPES at pH 7.4, 1 mM ATP, 30 mM creatine phosphate, 1 mM magnesium acetate, 0.15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1mM DTT), 500 nM bacterially expressed 6 × His-UBCs, 100 nM 6 × His-tagged human E1, 0.5 μM ubiquitin aldehyde in a volume of 15 ul.

  • Indeed, a time course of induction followed by western blotting of whole cell lysates using antibody to PP1α revealed diminution of the endogenous PP1α signal after 4 hr of induction, while the 6His-HA-PP1α signal increased (Figure 8).

  • Localization of this protein to the nucleus also mirrors that of endogenous PP1α in vivo . When using this system to forcibly overexpress 6His-HA-PP1α, there is a concomitant decrease in endogenous PP1α levels, suggesting the existence of an autoregulatory mechanism by which PP1α protein levels remain constant.

  • Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).

  • If mRNA association with ribosomes is truly a measure of translation efficiency, one conclusion from these data is that in doxycycline-induced cells, 6His-HA-PP1α RNA is more efficiently translated than endogenous PP1α.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast