Example sentences for: his-

How can you use “his-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1 His-C (control) was mixed with 50 μl of serum-free RPMI (Roswell Park Memorial Institute) medium.

  • The plasmids HA-Ub and His-Ub were described previously [ 22 ] .

  • Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).

  • The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).

  • Immunoprecipitation using PP1α antibody followed by western blotting using the same anti-PP1α reveals a reciprocal relationship in PP1α abundance following doxycycline induction; endogenous PP1α levels decrease in response to increasing levels of 6His-HA-PP1α (top panel, compare left two lanes).


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