Words similar to hh-signaling
Example sentences for: hh-signaling
How can you use “hh-signaling” in a sentence? Here are some example sentences to help you improve your vocabulary:
These data show that the Hh-signaling inhibitors, but not structurally related inactive compounds, can significantly compete with the binding of the Hh agonist to Smo-expressing cells.
The two Hh-signaling antagonists, KAAD-cyclopamine and Cur61414, have shown decreased potency on Smo act-expressing cells, leading to the speculation that they may bind this mutant form of Smo less well than the wild-type form [ 11 17 ] . We sought to determine whether the Hh agonist binds Smo actwith a higher affinity, an observation seen with certain ligands and constitutively active mutants of GPCRs [ 29 ] . To perform this experiment, we isolated membranes from cells transfected with a cDNA construct encoding a tryptophan-to-leucine mutation at residue 539 (W539L) of murine Smo.
Most notably, the neural-plate explant assay has defined the concentration-dependent role that ventrally expressed Shh plays in opposing dorsally expressed bone morphogenetic proteins (BMPs) to pattern the neural tube [ 2 ] . The assay demonstrates that the Hh-signaling cascade can distinguish between small concentration differences in the Hh ligand to instruct the differentiation of specific neuronal cell types.
Through a 'chemical genetic' approach of identifying and studying the mechanism of action of small-molecule agonists (and antagonists), we hoped to uncover some of the complexities of the Hh-signaling system.
These results argue that the agonist activates the pathway downstream of the Hh-Ptc interaction while cyclopamine, Cur61414 and the agonist may act at a similar level in the Hh-signaling cascade.
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