Words similar to hh-signaling
Example sentences for: hh-signaling
How can you use “hh-signaling” in a sentence? Here are some example sentences to help you improve your vocabulary:
These results argue that the agonist activates the pathway downstream of the Hh-Ptc interaction while cyclopamine, Cur61414 and the agonist may act at a similar level in the Hh-signaling cascade.
Small-molecule modulators of growth-factor pathways have proven valuable in providing enhanced understanding of the intracellular events that occur subsequent to receptor activation, and in establishing the biological functions of these pathways [ 6 7 8 ] . In Hh signaling, multiple insights have been gained through the use of the plant-derived Hh antagonist cyclopamine [ 9 10 11 12 13 14 15 16 ] and a recently identified synthetic small-molecule Hh-signaling inhibitor, Cur61414 [ 17 ] . Interestingly, these specific inhibitors of Hh signaling appear to function downstream of Ptc but their precise molecular target(s) and mechanism of action are unknown.
Stable Hh-signaling reporter cell lines were established by G418 selection following transfection with a luciferase reporter plasmid [ 20 ] containing the neomycin-resistance gene.
These data show that the Hh-signaling inhibitors, but not structurally related inactive compounds, can significantly compete with the binding of the Hh agonist to Smo membranes.
Furthermore, this supports the data showing that the agonist can activate signaling in Shh -/-embryos (Figure 3) and suggests that the agonist function is not only downstream of the Hh protein but also independent of the endogenous Hh-signaling modulators, Tout veloux and HIP, that act via the Hh ligand [ 2 ] . The competition experiment with forskolin showed identical inhibition curves for Hh protein and the agonist, strongly suggesting that the action of the small molecule is upstream of the protein-kinase-A-sensitive step in the pathway.
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