Words similar to hff

Example sentences for: hff

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The fluorescent image of oxidized OxyBURST Green H 2 HFF BSA in the same cell was then recorded as shown in Figure 9c.
If this is the case, then lysosomes containing OxyBURST Green H 2 HFF BSA should have more alkaline pH.
Although the location of activated NADPH oxidase complex in phagolysosomes has been reported, the release of either H 2 O 2 or O 2 •- in situ upon stimulation with PMA has not been directly shown in live cells [ 10 11 ] . In order to directly measure the oxidative activity in lysosomes, I use a reduced fluorescein derivative that is conjugated with BSA, OxyBURST Green H 2 HFF BSA, as shown in Figure 1. Spectrofluorimetric analysis indicates that this reduced compound is non-fluorescent, and becomes fluorescent upon HRP-catalyzed oxidation.
This clearly eliminates the possibility that there is more OxyBURST Green H 2 HFF BSA accumulating in lysosome area 2. The experiment strongly supports the notion that the oxidative activity within the lysosomal population is heterogeneous.
Furthermore, cells were incubated with the OxyBURST Green H 2 HFF at 37°C for 10 min so that the reduced dye is transported primarily into endosomes rather than lysosomes.