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Example sentences for: hff

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In order to demonstrate this relationship in live cells, the oxidative activities and pH in lysosomes were simultaneously monitored by OxyBURST Green H 2 HFF BSA and LysoTracker Red DND-99 which is able to qualitatively indicate acidic lysosomes.
Briefly, cell monolayers were incubated with 200 μg/mL OxyBURST Green H 2 HFF BSA at 37°C for 2 hours, washed, and then incubated in fresh medium containing 200 nM PMA at 37°C for 0.5 hour.
Cells were then labeled with OxyBURST Green H 2 HFF BSA, followed by chase labeling without the reduced dye but in the presence or absence of 200 nM PMA.
To study the effect of pH on the fluorescence of oxidized OxyBURST Green H 2 HFF BSA and 5-(and -6)-carboxyfluorescein, 78 μg/mL of the oxidized dye or 4.2 μM 5-(and -6)-carboxyfluorescein were prepared in MES (4-morpholineethanesulfonic acid) buffer solutions containing 5 mM NaCl, 115 mM KCl, 1.2 mM MgSO 4 and 25 mM MES (pHs from 4.5 to 7.5).
As shown in Figure 2, oxidized OxyBURST Green H 2 HFF BSA distributed within the population of lysosomes where they co-localized with the fluorescent dextran.