Words similar to hepg
Example sentences for: hepg
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For transfections, 10 5HepG2 cells were plated in 24 well dishes with DMEM supplemented with 10% charcoal stripped serum and transfected using the calcium phosphate precipitation method as described [ 16 ] . The next morning, cells were washed with phosphate buffered saline and ligands were added.
PMA did not improve IFNg inducibility of MHCII in MSTO-211H and HepG2 cell lines (data not shown).
MSTO-211H, mesothelioma, cell line also showed a weak induction of MHCII in response to IFNg and HepG2, hepatocellular carcinoma, was completely non-inducible (data not shown).
Rat G6pc has HNF4α consensus binding sites [ 52 ] . In HeLa cells and HepG2 hepatoma cells, PUFA down regulated G6pc, possibly via inhibition of HNF 4α promoter activity [ 52 ] . In our current mouse experiment, the transcriptional effects of LC-PUFA on G6pc may thus have been HNF4α- or PPARα mediated.
A substantial percentage of human tumors lose the expression of the retinoblastoma tumor suppressor protein (Rb), important as a necessary condition for IFNg-mediated induction of MHCII [ 21 ] . Therefore, we wished to determine whether the poor IFNg inducibility of MHCII in LS1034, MSTO-211H and HepG2 cell lines could be explained by the loss of Rb.
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