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Example sentences for: hbab

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More importantly however, expression of HBAB in McNtcp.
HBAB, a member of the human aldo keto reductase gene family, has been shown to bind bile acids with high affinity (<1 μM) and is postulated to play a similar role as 3α-hydroxysteroid dehydrogenase in human hepatocytes [ 33 ] . Expression of HBAB in McNtcp.
Analysis of radiolabeled TCA uptake (Figure 9B) showed that expression of HBAB had minimal impact on bile acid uptake despite the fact that the range of HBAB protein abundance was up to 5-fold greater than that in the lowest HBAB expressing cell.
High level expression of HBAB had a negligible effect on the ability of the BN clones to take up or secrete bile acids, suggesting that HBAB has a minor role in the net transport of bile acids in these cells.
The human bile acid binder (HBAB) cDNA [ 37 ] was cloned into a derivative of pCep4 (Invitrogen Corp., Carlsbad, CA), that had been previously modified by deleting the EBNA 1 gene [ 38 ] . The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.