Words similar to harvesting

Example sentences for: harvesting

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The number of viable GC in the monolayer cultures was determined by first harvesting the attached cells with a trypsin-containing solution (mg/ml): NaCl 8; KCl 0.4; dextrose 1; NaHCO 3 0.6; ethylenediaminetetraacetic acid sodium salt (Na-EDTA) 0.2; trypsin 0.5, and then counting directly the number of viable cells using trypan blue exclusion and hemacytometry.
Design of microarray experiments, cell harvesting and total RNA extraction
Cells were then serum deprived for 36-40 h in DMEM supplemented as above except containing 0.5% calf serum before harvesting.
Elaborate rituals grew up around the preparation of the milpas (cornfields) and the planting and harvesting of the crop whose success depended on the coming of the annual rains.
Freshly isolated PBMCs (10 5 cells) were incubated with baculovirus-derived recombinant p24 protein (Protein Sciences, Meriden, Connecticut, United States) at 5 μg/ml for 6 d and then pulsed with 3 H thymidine at 1.0 μCi for 6 h before harvesting as previously described [20].