Example sentences for: harvested

How can you use “harvested” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were then harvested, and total RNA was extracted with TRIzol Reagent (GIBCO-BRL) according to the methods of the manufacturer.

  • After incubation, media and cells were harvested for analysis.

  • Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).

  • To assess chemokine mRNA expression, cell lines were harvested after 4 h of culture, then suspended in Trizol and stored at -80°C.

  • The cells were harvested by trypsinization, sonicated, and incubated for 1 hr in 10% tri-chloro-acetic acid (TCA) on ice.


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