Words similar to harvested

Example sentences for: harvested

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Briefly, cells were washed and harvested with ice cold phosphate buffered saline.
To test whether the Hh agonist could interact with Smo in vitro , we transiently overexpressed murine Smo, murine Ptc, rat β2-adrenergic receptor and GFP in 293T cells, harvested membranes and performed a filtration membrane-binding assay in a 96-well plate with [ 3H]-Hh-Ag 1.5 added at 2 nM.
Seedlings were harvested when 7 days old.
After treatment, media were harvested, and sAPP α levels were evaluated by immunoblotting as described above.
Subcellular fractionation of RNA into cytoskeleton-associated and cytosolic fractions was performed as previously described [ 15 34 ] . In brief, HL-60 cells were harvested by centrifugation and resuspension in buffer A (10 mM Tris pH 7.6, 0.25 M sucrose, 25 mM KCl, 5 mM MgCl 2 ) with 0.1% Igepal nonionic detergent (Sigma).